AAE15_ARATH
ID AAE15_ARATH Reviewed; 727 AA.
AC Q8W471; O23268; Q56X36; Q8LRT2;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Long-chain-fatty-acid--[acyl-carrier-protein] ligase AEE15, chloroplastic;
DE EC=6.2.1.20;
DE AltName: Full=Acyl-[acyl-carrier-protein] synthetase;
DE AltName: Full=Acyl-activating enzyme 15;
DE Flags: Precursor;
GN Name=AAE15; OrderedLocusNames=At4g14070; ORFNames=dl3075c, FCAALL.81;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=16262711; DOI=10.1111/j.1365-313x.2005.02553.x;
RA Koo A.J., Fulda M., Browse J., Ohlrogge J.B.;
RT "Identification of a plastid acyl-acyl carrier protein synthetase in
RT Arabidopsis and its role in the activation and elongation of exogenous
RT fatty acids.";
RL Plant J. 44:620-632(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 19-727.
RX PubMed=12177484; DOI=10.1104/pp.003269;
RA Shockey J.M., Fulda M.S., Browse J.A.;
RT "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that
RT participate in fatty acid and glycerolipid metabolism.";
RL Plant Physiol. 129:1710-1722(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 321-727.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12805634; DOI=10.1104/pp.103.020552;
RA Shockey J.M., Fulda M.S., Browse J.;
RT "Arabidopsis contains a large superfamily of acyl-activating enzymes.
RT Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme
RT a synthetases.";
RL Plant Physiol. 132:1065-1076(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Probably involved in the activation of fatty acids to acyl-
CC carrier-protein prior to fatty acid elongation in plastids. Acts on
CC medium- to long-chain fatty acids. {ECO:0000269|PubMed:16262711}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain fatty acid + ATP + holo-[ACP] = a long-chain
CC fatty acyl-[ACP] + AMP + diphosphate; Xref=Rhea:RHEA:45588,
CC Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:12682, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57560, ChEBI:CHEBI:64479,
CC ChEBI:CHEBI:133243, ChEBI:CHEBI:456215; EC=6.2.1.20;
CC Evidence={ECO:0000269|PubMed:16262711};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD94085.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB10186.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78449.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; EF093797; ABK88270.1; -; mRNA.
DR EMBL; Z97335; CAB10186.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161538; CAB78449.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83367.1; -; Genomic_DNA.
DR EMBL; AY062804; AAL32882.1; -; mRNA.
DR EMBL; BT010332; AAQ56775.1; -; mRNA.
DR EMBL; AF503770; AAM28628.1; -; mRNA.
DR EMBL; AK221841; BAD94085.1; ALT_INIT; mRNA.
DR PIR; H71401; H71401.
DR RefSeq; NP_193143.2; NM_117482.4.
DR AlphaFoldDB; Q8W471; -.
DR STRING; 3702.AT4G14070.1; -.
DR iPTMnet; Q8W471; -.
DR PaxDb; Q8W471; -.
DR PRIDE; Q8W471; -.
DR ProMEX; Q8W471; -.
DR ProteomicsDB; 245131; -.
DR EnsemblPlants; AT4G14070.1; AT4G14070.1; AT4G14070.
DR GeneID; 827043; -.
DR Gramene; AT4G14070.1; AT4G14070.1; AT4G14070.
DR KEGG; ath:AT4G14070; -.
DR Araport; AT4G14070; -.
DR TAIR; locus:2129371; AT4G14070.
DR eggNOG; KOG1256; Eukaryota.
DR HOGENOM; CLU_000022_45_5_1; -.
DR InParanoid; Q8W471; -.
DR OMA; GDTGWIA; -.
DR OrthoDB; 806831at2759; -.
DR PhylomeDB; Q8W471; -.
DR BioCyc; ARA:AT4G14070-MON; -.
DR BRENDA; 6.2.1.20; 399.
DR PRO; PR:Q8W471; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8W471; baseline and differential.
DR Genevisible; Q8W471; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009536; C:plastid; IDA:TAIR.
DR GO; GO:0008922; F:long-chain fatty acid [acyl-carrier-protein] ligase activity; IMP:TAIR.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IMP:TAIR.
DR GO; GO:0030497; P:fatty acid elongation; IDA:TAIR.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR Pfam; PF00501; AMP-binding; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Fatty acid metabolism; Ligase; Lipid metabolism; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..66
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 67..727
FT /note="Long-chain-fatty-acid--[acyl-carrier-protein] ligase
FT AEE15, chloroplastic"
FT /id="PRO_0000415725"
FT CONFLICT 318
FT /note="Q -> L (in Ref. 6; AAM28628)"
FT /evidence="ECO:0000305"
FT CONFLICT 595
FT /note="G -> A (in Ref. 6; AAM28628)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 727 AA; 81466 MW; 059BBC287AB9F1C2 CRC64;
MQIRLKPDYS FFIASSSTMA STSSLGPSTL LSYGSPSRQF PDFGFRLISG HESVRIPSFR
RFRVHCESKE KEVKPSSPFL ESSSFSGDAA LRSSEWKAVP DIWRSSAEKY GDRVALVDPY
HDPPLKLTYK QLEQEILDFA EGLRVLGVKA DEKIALFADN SCRWLVSDQG IMATGAVNVV
RGSRSSVEEL LQIYRHSESV AIVVDNPEFF NRIAESFTSK ASLRFLILLW GEKSSLVTQG
MQIPVYSYAE IINQGQESRA KLSASNDTRS YRNQFIDSDD TAAIMYTSGT TGNPKGVMLT
HRNLLHQIKH LSKYVPAQAG DKFLSMLPSW HAYERASEYF IFTCGVEQMY TSIRYLKDDL
KRYQPNYIVS VPLVYETLYS GIQKQISASS AGRKFLALTL IKVSMAYMEM KRIYEGMCLT
KEQKPPMYIV AFVDWLWARV IAALLWPLHM LAKKLIYKKI HSSIGISKAG ISGGGSLPIH
VDKFFEAIGV ILQNGYGLTE TSPVVCARTL SCNVLGSAGH PMHGTEFKIV DPETNNVLPP
GSKGIIKVRG PQVMKGYYKN PSTTKQVLNE SGWFNTGDTG WIAPHHSKGR SRHCGGVIVL
EGRAKDTIVL STGENVEPLE IEEAAMRSRV IEQIVVIGQD RRRLGAIIIP NKEEAQRVDP
ETSKETLKSL VYQELRKWTS ECSFQVGPVL IVDDPFTIDN GLMTPTMKIR RDMVVAKYKE
EIDQLYS
