PUR7_ARATH
ID PUR7_ARATH Reviewed; 411 AA.
AC P38025; Q8RXX4; Q9LJC1;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2002, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase, chloroplastic;
DE EC=6.3.2.6;
DE AltName: Full=SAICAR synthetase;
DE Flags: Precursor;
GN Name=PUR7; OrderedLocusNames=At3g21110; ORFNames=MSA6.15, MSA6.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia; TISSUE=Leaf;
RX PubMed=8938402; DOI=10.1104/pp.112.3.905;
RA Senecoff J.F., McKinney E.C., Meagher R.B.;
RT "De novo purine synthesis in Arabidopsis thaliana. II. The PUR7 gene
RT encoding 5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole
RT synthetase is expressed in rapidly dividing tissues.";
RL Plant Physiol. 112:905-917(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP +
CC L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-
CC 4-carboxamido]succinate + ADP + 2 H(+) + phosphate;
CC Xref=Rhea:RHEA:22628, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58443,
CC ChEBI:CHEBI:77657, ChEBI:CHEBI:456216; EC=6.3.2.6;
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-
CC phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the SAICAR synthetase family. {ECO:0000305}.
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DR EMBL; U05599; AAA16231.1; -; Unassigned_DNA.
DR EMBL; AP000604; BAB01454.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76462.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76463.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63435.1; -; Genomic_DNA.
DR EMBL; AY080627; AAL85973.1; -; mRNA.
DR EMBL; BT002734; AAO22563.1; -; mRNA.
DR RefSeq; NP_001030739.1; NM_001035662.2.
DR RefSeq; NP_001319605.1; NM_001338505.1.
DR RefSeq; NP_188748.1; NM_113005.4.
DR AlphaFoldDB; P38025; -.
DR SMR; P38025; -.
DR STRING; 3702.AT3G21110.1; -.
DR PaxDb; P38025; -.
DR PRIDE; P38025; -.
DR ProteomicsDB; 226088; -.
DR EnsemblPlants; AT3G21110.1; AT3G21110.1; AT3G21110.
DR EnsemblPlants; AT3G21110.2; AT3G21110.2; AT3G21110.
DR EnsemblPlants; AT3G21110.3; AT3G21110.3; AT3G21110.
DR GeneID; 821663; -.
DR Gramene; AT3G21110.1; AT3G21110.1; AT3G21110.
DR Gramene; AT3G21110.2; AT3G21110.2; AT3G21110.
DR Gramene; AT3G21110.3; AT3G21110.3; AT3G21110.
DR KEGG; ath:AT3G21110; -.
DR Araport; AT3G21110; -.
DR TAIR; locus:2092975; AT3G21110.
DR eggNOG; KOG2835; Eukaryota.
DR HOGENOM; CLU_045637_2_0_1; -.
DR InParanoid; P38025; -.
DR OMA; TKFEFGF; -.
DR OrthoDB; 566769at2759; -.
DR PhylomeDB; P38025; -.
DR BioCyc; ARA:AT3G21110-MON; -.
DR UniPathway; UPA00074; UER00131.
DR PRO; PR:P38025; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; P38025; baseline and differential.
DR Genevisible; P38025; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IBA:GO_Central.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IBA:GO_Central.
DR GO; GO:0009733; P:response to auxin; IDA:TAIR.
DR HAMAP; MF_00137; SAICAR_synth; 1.
DR InterPro; IPR028923; SAICAR_synt/ADE2_N.
DR InterPro; IPR018236; SAICAR_synthetase_CS.
DR Pfam; PF01259; SAICAR_synt; 1.
DR PROSITE; PS01057; SAICAR_SYNTHETASE_1; 1.
DR PROSITE; PS01058; SAICAR_SYNTHETASE_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Ligase; Nucleotide-binding; Plastid;
KW Purine biosynthesis; Reference proteome; Transit peptide.
FT TRANSIT 1..53
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 54..411
FT /note="Phosphoribosylaminoimidazole-succinocarboxamide
FT synthase, chloroplastic"
FT /id="PRO_0000029873"
FT CONFLICT 91
FT /note="S -> C (in Ref. 1; AAA16231)"
FT /evidence="ECO:0000305"
FT CONFLICT 168..170
FT /note="IVS -> MLY (in Ref. 1; AAA16231)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 411 AA; 46063 MW; A338C8B6E02E9A0C CRC64;
MAQCVRSTLN PVRTPQSFTR KAYVKSPAFA SVSFLRAVPE FNKYPKPCSL VMSCQGKAQN
QQEERPQLSL DDLVTSNRKG EVLGTIKDSL SNCLSETNLL ATVPGLKSRI KGKVRDIYDA
GDYLVLITTD RLSAFDRNLA SIPFKGQVLN ETSLWWFNNT QHITPNAIVS SPDRNVVIAK
KCSVFPIEFV VRGYVTGSTD TSLWTVYNKG VRNYCGNELS DGLVKNQKLP ANILTPTTKA
ADHDVPISPN EIVEGGFMTQ AEFDEASMKA LSLFEFGQGV AKKHGLILVD TKYEFGRSSD
GSILLIDEIH TPDSSRYWLA GSYEERFQKG LEPENVDKEF LRLWFKENCN PYEDEVLPAA
PAELVTELAW RYIFLYETIT GSRIDIIPTQ EPIHDRISRN TSQALSSLRQ L
