ID   PUR7_CYBJA              Reviewed;         301 AA.
AC   P50124;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase;
DE            EC=6.3.2.6;
DE   AltName: Full=SAICAR synthetase;
GN   Name=ADE1;
OS   Cyberlindnera jadinii (Torula yeast) (Pichia jadinii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Cyberlindnera.
OX   NCBI_TaxID=4903;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CA(U)-37;
RX   PubMed=7764516; DOI=10.1271/bbb.58.208;
RA   Nishiya Y.;
RT   "Primary structure of the ADE1 gene from Candida utilis.";
RL   Biosci. Biotechnol. Biochem. 58:208-210(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP +
CC         L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-
CC         4-carboxamido]succinate + ADP + 2 H(+) + phosphate;
CC         Xref=Rhea:RHEA:22628, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58443,
CC         ChEBI:CHEBI:77657, ChEBI:CHEBI:456216; EC=6.3.2.6;
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC       amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-
CC       phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
CC   -!- SIMILARITY: Belongs to the SAICAR synthetase family. {ECO:0000305}.
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DR   EMBL; D17356; BAA04175.1; -; Genomic_DNA.
DR   PIR; JC2039; JC2039.
DR   AlphaFoldDB; P50124; -.
DR   SMR; P50124; -.
DR   UniPathway; UPA00074; UER00131.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00137; SAICAR_synth; 1.
DR   InterPro; IPR028923; SAICAR_synt/ADE2_N.
DR   InterPro; IPR001636; SAICAR_synth.
DR   InterPro; IPR018236; SAICAR_synthetase_CS.
DR   Pfam; PF01259; SAICAR_synt; 1.
DR   TIGRFAMs; TIGR00081; purC; 1.
DR   PROSITE; PS01057; SAICAR_SYNTHETASE_1; 1.
DR   PROSITE; PS01058; SAICAR_SYNTHETASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; Purine biosynthesis.
FT   CHAIN           1..301
FT                   /note="Phosphoribosylaminoimidazole-succinocarboxamide
FT                   synthase"
FT                   /id="PRO_0000100926"
SQ   SEQUENCE   301 AA;  33797 MW;  454BD14F94E78E06 CRC64;
     MSITQTNLDN ILPLVARGKV RDIYEVDEKT LLFVATDRIS AYDVIMENGI PQKGQLLTQL
     SEFWFEFLKP YIKTHLIEVD DIFAHLPKVL SEDRYKSQLQ GGSLLVRKYK LVPLEVIVRG
     YITGSAWKVY KKSGTVHGVK AAEGLQESQE FPTPIFTPST KAEQGEHDEN ISVEQAEAIV
     GKELADKIGK VAVELYSKAK GYAAGRGIII ADTKFEFGLD DDNELVLVDE VLTPDSSRFW
     NAKTYEVGKG QDSYDKQFLR DWLTSNGLAG KEGVSMTEDI ATRSRVKYVE AYEALTGKKW
     E