PUR7_METJA
ID PUR7_METJA Reviewed; 242 AA.
AC Q58987;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase;
DE EC=6.3.2.6;
DE AltName: Full=SAICAR synthetase;
GN Name=purC; OrderedLocusNames=MJ1592;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP +
CC L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-
CC 4-carboxamido]succinate + ADP + 2 H(+) + phosphate;
CC Xref=Rhea:RHEA:22628, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58443,
CC ChEBI:CHEBI:77657, ChEBI:CHEBI:456216; EC=6.3.2.6;
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-
CC phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
CC -!- SIMILARITY: Belongs to the SAICAR synthetase family. {ECO:0000305}.
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DR EMBL; L77117; AAB99610.1; -; Genomic_DNA.
DR PIR; G64498; G64498.
DR RefSeq; WP_010871116.1; NC_000909.1.
DR PDB; 2YZL; X-ray; 2.20 A; A=1-242.
DR PDB; 2Z02; X-ray; 2.03 A; A/B=1-242.
DR PDBsum; 2YZL; -.
DR PDBsum; 2Z02; -.
DR AlphaFoldDB; Q58987; -.
DR SMR; Q58987; -.
DR STRING; 243232.MJ_1592; -.
DR PRIDE; Q58987; -.
DR EnsemblBacteria; AAB99610; AAB99610; MJ_1592.
DR GeneID; 1452500; -.
DR KEGG; mja:MJ_1592; -.
DR eggNOG; arCOG04421; Archaea.
DR HOGENOM; CLU_061495_2_0_2; -.
DR InParanoid; Q58987; -.
DR OMA; EFCYKND; -.
DR OrthoDB; 47738at2157; -.
DR PhylomeDB; Q58987; -.
DR BRENDA; 6.3.2.6; 3260.
DR UniPathway; UPA00074; UER00131.
DR EvolutionaryTrace; Q58987; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:InterPro.
DR CDD; cd01415; SAICAR_synt_PurC; 1.
DR HAMAP; MF_00137; SAICAR_synth; 1.
DR InterPro; IPR028923; SAICAR_synt/ADE2_N.
DR InterPro; IPR033934; SAICAR_synt_PurC.
DR InterPro; IPR001636; SAICAR_synth.
DR InterPro; IPR018236; SAICAR_synthetase_CS.
DR Pfam; PF01259; SAICAR_synt; 1.
DR TIGRFAMs; TIGR00081; purC; 1.
DR PROSITE; PS01057; SAICAR_SYNTHETASE_1; 1.
DR PROSITE; PS01058; SAICAR_SYNTHETASE_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Ligase; Nucleotide-binding; Purine biosynthesis;
KW Reference proteome.
FT CHAIN 1..242
FT /note="Phosphoribosylaminoimidazole-succinocarboxamide
FT synthase"
FT /id="PRO_0000100909"
FT HELIX 5..8
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 14..16
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 18..24
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 26..34
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 36..40
FT /evidence="ECO:0007829|PDB:2Z02"
FT TURN 41..44
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 45..48
FT /evidence="ECO:0007829|PDB:2Z02"
FT HELIX 52..69
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 74..80
FT /evidence="ECO:0007829|PDB:2Z02"
FT TURN 81..83
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 84..88
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 90..92
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 94..102
FT /evidence="ECO:0007829|PDB:2Z02"
FT HELIX 106..110
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 118..128
FT /evidence="ECO:0007829|PDB:2Z02"
FT HELIX 131..133
FT /evidence="ECO:0007829|PDB:2Z02"
FT HELIX 140..145
FT /evidence="ECO:0007829|PDB:2Z02"
FT HELIX 151..174
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 177..184
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 186..189
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 194..196
FT /evidence="ECO:0007829|PDB:2Z02"
FT TURN 202..204
FT /evidence="ECO:0007829|PDB:2Z02"
FT STRAND 205..209
FT /evidence="ECO:0007829|PDB:2Z02"
FT TURN 210..212
FT /evidence="ECO:0007829|PDB:2Z02"
FT HELIX 219..222
FT /evidence="ECO:0007829|PDB:2Z02"
FT HELIX 228..238
FT /evidence="ECO:0007829|PDB:2Z02"
SQ SEQUENCE 242 AA; 27761 MW; E72A473335DA3E1E CRC64;
MEIKLEEILK KQPLYSGKAK SIYEIDDDKV LIEFRDDITA GNGAKHDVKQ GKGYLNALIS
SKLFEALEEN GVKTHYIKYI EPRYMIAKKV EIIPIEVIVR NIAAGSLCRR YPFEEGKELP
FPIVQFDYKN DEYGDPMLNE DIAVALGLAT REELNKIKEI ALKVNEVLKK LFDEKGIILV
DFKIEIGKDR EGNLLVADEI SPDTMRLWDK ETRDVLDKDV FRKDLGDVIA KYRIVAERLG
LL
