ID   PUR7_MYCTU              Reviewed;         297 AA.
AC   P9WHN1; L0T7P9; P0A5T4; P77904; Q59566;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase;
DE            EC=6.3.2.6;
DE   AltName: Full=SAICAR synthetase;
GN   Name=purC; OrderedLocusNames=Rv0780; ORFNames=MTCY369.24;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=8828210; DOI=10.1099/00221287-142-9-2439;
RA   Jackson M., Berthet F.-X., Otal I., Rauzier J., Martin C., Gicquel B.,
RA   Guilhot C.;
RT   "The Mycobacterium tuberculosis purine biosynthetic pathway: isolation and
RT   characterization of the purC and purL genes.";
RL   Microbiology 142:2439-2447(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP +
CC         L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-
CC         4-carboxamido]succinate + ADP + 2 H(+) + phosphate;
CC         Xref=Rhea:RHEA:22628, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58443,
CC         ChEBI:CHEBI:77657, ChEBI:CHEBI:456216; EC=6.3.2.6;
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC       amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-
CC       phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
CC   -!- SIMILARITY: Belongs to the SAICAR synthetase family. {ECO:0000305}.
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DR   EMBL; U34957; AAB41456.1; -; Genomic_DNA.
DR   EMBL; AL123456; CCP43527.1; -; Genomic_DNA.
DR   PIR; G70708; G70708.
DR   RefSeq; NP_215294.1; NC_000962.3.
DR   RefSeq; WP_003403962.1; NZ_NVQJ01000035.1.
DR   AlphaFoldDB; P9WHN1; -.
DR   SMR; P9WHN1; -.
DR   STRING; 83332.Rv0780; -.
DR   PaxDb; P9WHN1; -.
DR   DNASU; 888928; -.
DR   GeneID; 888928; -.
DR   KEGG; mtu:Rv0780; -.
DR   TubercuList; Rv0780; -.
DR   eggNOG; COG0152; Bacteria.
DR   OMA; TKFEFGF; -.
DR   PhylomeDB; P9WHN1; -.
DR   UniPathway; UPA00074; UER00131.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IBA:GO_Central.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006164; P:purine nucleotide biosynthetic process; IMP:MTBBASE.
DR   HAMAP; MF_00137; SAICAR_synth; 1.
DR   InterPro; IPR028923; SAICAR_synt/ADE2_N.
DR   InterPro; IPR001636; SAICAR_synth.
DR   InterPro; IPR018236; SAICAR_synthetase_CS.
DR   Pfam; PF01259; SAICAR_synt; 1.
DR   TIGRFAMs; TIGR00081; purC; 1.
DR   PROSITE; PS01057; SAICAR_SYNTHETASE_1; 1.
DR   PROSITE; PS01058; SAICAR_SYNTHETASE_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Ligase; Nucleotide-binding; Purine biosynthesis;
KW   Reference proteome.
FT   CHAIN           1..297
FT                   /note="Phosphoribosylaminoimidazole-succinocarboxamide
FT                   synthase"
FT                   /id="PRO_0000100843"
FT   CONFLICT        124
FT                   /note="A -> R (in Ref. 1; AAB41456)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        166
FT                   /note="A -> P (in Ref. 1; AAB41456)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   297 AA;  32930 MW;  200D1EA37DF98637 CRC64;
     MRPALSDYQH VASGKVREIY RVDDEHLLLV ASDRISAYDY VLDSTIPDKG RVLTAMSAFF
     FGLVDAPNHL AGPPDDPRIP DEVLGRALVV RRLEMLPVEC VARGYLTGSG LLDYQATGKV
     CGIALPPGLV EASRFATPLF TPATKAALGD HDENISFDRV VEMVGALRAN QLRDRTLQTY
     VQAADHALTR GIIIADTKFE FGIDRHGNLL LADEIFTPDS SRYWPADDYR AGVVQTSFDK
     QFVRSWLTGS ESGWDRGSDR PPPPLPEHIV EATRARYINA YERISELKFD DWIGPGA