ID   PUR7_PICPA              Reviewed;         304 AA.
AC   Q9C1J4;
DT   26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Phosphoribosylaminoimidazole-succinocarboxamide synthase;
DE            EC=6.3.2.6;
DE   AltName: Full=SAICAR synthetase;
GN   Name=ADE1;
OS   Komagataella pastoris (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=4922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11223254; DOI=10.1016/s0378-1119(00)00576-x;
RA   Lin Cereghino G.P., Lin Cereghino J., Sunga A.J., Johnson M.A., Lim M.,
RA   Gleeson M.A.G., Cregg J.M.;
RT   "New selectable marker/auxotrophic host strain combinations for molecular
RT   genetic manipulation of Pichia pastoris.";
RL   Gene 263:159-169(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP +
CC         L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-
CC         4-carboxamido]succinate + ADP + 2 H(+) + phosphate;
CC         Xref=Rhea:RHEA:22628, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58443,
CC         ChEBI:CHEBI:77657, ChEBI:CHEBI:456216; EC=6.3.2.6;
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC       amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-
CC       phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
CC   -!- SIMILARITY: Belongs to the SAICAR synthetase family. {ECO:0000305}.
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DR   EMBL; AF321096; AAK06766.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9C1J4; -.
DR   SMR; Q9C1J4; -.
DR   UniPathway; UPA00074; UER00131.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004639; F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00137; SAICAR_synth; 1.
DR   InterPro; IPR028923; SAICAR_synt/ADE2_N.
DR   InterPro; IPR001636; SAICAR_synth.
DR   InterPro; IPR018236; SAICAR_synthetase_CS.
DR   Pfam; PF01259; SAICAR_synt; 1.
DR   TIGRFAMs; TIGR00081; purC; 1.
DR   PROSITE; PS01057; SAICAR_SYNTHETASE_1; 1.
DR   PROSITE; PS01058; SAICAR_SYNTHETASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; Purine biosynthesis.
FT   CHAIN           1..304
FT                   /note="Phosphoribosylaminoimidazole-succinocarboxamide
FT                   synthase"
FT                   /id="PRO_0000100927"
SQ   SEQUENCE   304 AA;  34101 MW;  584073DA712262C9 CRC64;
     MSIVNTDLDG ILPLIAKGKV RDIYAVDENN LLFVATDRIS AYDVIMTNGI PDKGKILTQL
     SVFWFDFLAP YIKNHLVASN DKEVFALLPS KLSEEKYKSQ LEGRSLIVKK HRLIPLEAIV
     RGYITGSAWK EYKNSKTVHG VKVENENLQE SDAFPTPIFT PSTKAEQGEH DENISIEQAA
     EIVGKDICEK VAVKAVELYS AAKNFALLKG IIIADTKFEF GLDENNELVL VDEVLTPDSS
     RFWNQKTYQV GKSQESYDKQ FLRDWLTANG LNGKEGVAMD AEIAIKSKEK YIEAYEAITG
     KKWA