AAE20_ARATH
ID AAE20_ARATH Reviewed; 580 AA.
AC Q9SS01;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Benzoate--CoA ligase, peroxisomal;
DE EC=6.2.1.25;
DE AltName: Full=Acyl-activating enzyme 20;
DE AltName: Full=Protein BENZOYLOXYGLUCOSINOLATE 1;
GN Name=AAE20; Synonyms=BZO1; OrderedLocusNames=At1g65880; ORFNames=F12P19.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12805634; DOI=10.1104/pp.103.020552;
RA Shockey J.M., Fulda M.S., Browse J.;
RT "Arabidopsis contains a large superfamily of acyl-activating enzymes.
RT Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme
RT a synthetases.";
RL Plant Physiol. 132:1065-1076(2003).
RN [4]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RC STRAIN=cv. Columbia;
RX PubMed=17651367; DOI=10.1111/j.1365-313x.2007.03205.x;
RA Kliebenstein D.J., D'Auria J.C., Behere A.S., Kim J.H., Gunderson K.L.,
RA Breen J.N., Lee G., Gershenzon J., Last R.L., Jander G.;
RT "Characterization of seed-specific benzoyloxyglucosinolate mutations in
RT Arabidopsis thaliana.";
RL Plant J. 51:1062-1076(2007).
CC -!- FUNCTION: Benzoate--CoA ligase involved in benzoyloxyglucosinolate
CC biosynthesis in seeds. Glucosinolates are secondary metabolites
CC involved in pathogen and insect defense of cruciferous plants.
CC {ECO:0000269|PubMed:17651367}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + benzoate + CoA = AMP + benzoyl-CoA + diphosphate;
CC Xref=Rhea:RHEA:10132, ChEBI:CHEBI:16150, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57369,
CC ChEBI:CHEBI:456215; EC=6.2.1.25;
CC Evidence={ECO:0000269|PubMed:17651367};
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
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DR EMBL; AC009513; AAF06049.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34436.1; -; Genomic_DNA.
DR PIR; A96683; A96683.
DR RefSeq; NP_176763.1; NM_105260.3.
DR AlphaFoldDB; Q9SS01; -.
DR SMR; Q9SS01; -.
DR STRING; 3702.AT1G65880.1; -.
DR PaxDb; Q9SS01; -.
DR PRIDE; Q9SS01; -.
DR ProteomicsDB; 244624; -.
DR EnsemblPlants; AT1G65880.1; AT1G65880.1; AT1G65880.
DR GeneID; 842900; -.
DR Gramene; AT1G65880.1; AT1G65880.1; AT1G65880.
DR KEGG; ath:AT1G65880; -.
DR Araport; AT1G65880; -.
DR TAIR; locus:2009774; AT1G65880.
DR eggNOG; KOG1176; Eukaryota.
DR HOGENOM; CLU_000022_59_5_1; -.
DR InParanoid; Q9SS01; -.
DR OMA; AWPNTDF; -.
DR OrthoDB; 312083at2759; -.
DR PhylomeDB; Q9SS01; -.
DR BioCyc; ARA:AT1G65880-MON; -.
DR BioCyc; MetaCyc:AT1G65880-MON; -.
DR PRO; PR:Q9SS01; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SS01; baseline and differential.
DR Genevisible; Q9SS01; AT.
DR GO; GO:0005777; C:peroxisome; IDA:TAIR.
DR GO; GO:0018858; F:benzoate-CoA ligase activity; IDA:TAIR.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0019761; P:glucosinolate biosynthetic process; IMP:TAIR.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
PE 1: Evidence at protein level;
KW Ligase; Peroxisome; Plant defense; Reference proteome.
FT CHAIN 1..580
FT /note="Benzoate--CoA ligase, peroxisomal"
FT /id="PRO_0000415730"
FT MOTIF 578..580
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
SQ SEQUENCE 580 AA; 64941 MW; 3855327A59D6E3A7 CRC64;
MDDLALCEAN NVPLTPMTFL KRASECYPNR TSIIYGKTRF TWPQTYDRCC RLAASLISLN
ISKNDVVSVM APNTPALYEM HFAVPMAGAV LNPINTRLDA TSIAAILRHA KPKILFLDRS
FEALARESLH LLSSEDSNLN LPVIFIHEND FPKRASFEEL DYECLIQRGE PTPSMVARMF
RIQDEHDPIS LNYTSGTTAD PKGVVISHRG AYLCTLSAII GWEMGTCPVY LWTLPMFHCN
GWTFTWGTAA RGGTSVCMRH VTAPEIYKNI EMHNVTHMCC VPTVFNILLK GNSLDLSPRS
GPVHVLTGGS PPPAALVKKV QRLGFQVMHA YGQTEATGPI LFCEWQDEWN RLPENQQMEL
KARQGISILG LADVDVKNKE TQKSAPRDGK TMGEILIKGS SIMKGYLKNP KATFEAFKHG
WLNTGDVGVI HPDGHVEIKD RSKDIIISGG ENISSVEVEN VLYKYPKVLE TAVVAMPHPT
WGETPCAFVV LEKSETTIKE DRVDKFQTRE RNLIEYCREN LPHFMCPRKV VFLEELPKNG
NGKILKPKLR DIAKGLVVED EINVIAKEVK RPVGHFISRL
