ID   PUR8_STRAD              Reviewed;         503 AA.
AC   P42670;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Puromycin resistance protein pur8;
GN   Name=pur8;
OS   Streptomyces alboniger.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces aurantiacus group.
OX   NCBI_TaxID=132473;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 12461 / DSM 40043 / JCM 4309 / NBRC 12738 / NCIMB 13007 / NRRL
RC   B-2403;
RX   PubMed=7916693; DOI=10.1111/j.1432-1033.1993.tb18454.x;
RA   Tercero J.A., Lacalle R.A., Jimenez A.;
RT   "The pur8 gene from the pur cluster of Streptomyces alboniger encodes a
RT   highly hydrophobic polypeptide which confers resistance to puromycin.";
RL   Eur. J. Biochem. 218:963-971(1993).
CC   -!- FUNCTION: May be involved in active puromycin efflux energized by a
CC       proton-dependent electrochemical gradient. In addition, it could be
CC       implicated in secreting N-acetylpuromycin, the last intermediate of the
CC       puromycin biosynthesis pathway, to the environment.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. EmrB family.
CC       {ECO:0000305}.
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DR   EMBL; X76855; CAA54186.1; -; Genomic_DNA.
DR   AlphaFoldDB; P42670; -.
DR   SMR; P42670; -.
DR   TCDB; 2.A.1.3.11; the major facilitator superfamily (mfs).
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Antibiotic resistance; Cell membrane; Membrane;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..503
FT                   /note="Puromycin resistance protein pur8"
FT                   /id="PRO_0000173399"
FT   TOPO_DOM        1..24
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        46..64
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        86..92
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        114..122
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        144..152
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        174..181
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        182..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        203..212
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        213..233
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        234..241
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        242..262
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        263..280
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        281..301
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        302..313
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        314..334
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        335..346
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        347..367
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        368..371
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        372..392
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        393..422
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        423..443
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        444..461
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        462..482
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        483..503
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   503 AA;  51852 MW;  FA3B814DF9A3EB74 CRC64;
     MARKPDISAV PVESAACQGP DPRRWWGLVV ILAAQLLVVL DGTVVNIALP SVQRDLGMSD
     TSRQWVITAY TLAFGGLLLL GGRVADAFGR RRIFAVGILG FGLASLLGGA APDPGTLFLA
     RALQGVFAAA LAPAALALIN TLFTEPGERG KAFGVYGAVS GGGAAVGLLA GGLLTEYLDW
     RWCLYVNAPV ALLALLGCRL LPRDRRTGRA VRLDLPGTLL GCGGLVAIVY AFAEAESGWG
     DPLVVRLLVL GVLMLVAFAL VERRVQDPLL PPGVVAHRVR GGSFLVVGLP QIGLFGLFLF
     LTYYLQGILD YSPVLTGVAF LPLGLGIAVG SSLIAARLLP RTRPRTLIVG ALLAAAAGMA
     LLTRLEPDTP QVYLTHLLPA QILIGLGIGC MMMPAMHTAT ARVAPHEAGA AAAVVNSAQQ
     VGGALGVALL NTVSTGATAA YLADHGTSPA ATVDGTVHGY TVAIAFAVGV LLLTAVLAWV
     LIDSRTEAAD ETGSASVTPA RPR