AAE4_ARATH
ID AAE4_ARATH Reviewed; 545 AA.
AC O80658; Q8LRT5;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Probable acyl-activating enzyme 4;
DE EC=6.2.1.-;
DE AltName: Full=AMP-binding protein 4;
DE Short=AtAMPBP4;
GN Name=AEE4; Synonyms=AMPBP4; OrderedLocusNames=At1g77240; ORFNames=T14N5.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12177484; DOI=10.1104/pp.003269;
RA Shockey J.M., Fulda M.S., Browse J.A.;
RT "Arabidopsis contains nine long-chain acyl-coenzyme A synthetase genes that
RT participate in fatty acid and glycerolipid metabolism.";
RL Plant Physiol. 129:1710-1722(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12805634; DOI=10.1104/pp.103.020552;
RA Shockey J.M., Fulda M.S., Browse J.;
RT "Arabidopsis contains a large superfamily of acyl-activating enzymes.
RT Phylogenetic and biochemical analysis reveals a new class of acyl-coenzyme
RT a synthetases.";
RL Plant Physiol. 132:1065-1076(2003).
CC -!- FUNCTION: May act as an acid--thiol ligase that activates carboxylic
CC acids by forming acyl-CoAs. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, leaves, stems, flowers and
CC developing seeds. {ECO:0000269|PubMed:12805634}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC {ECO:0000305}.
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DR EMBL; AF503763; AAM28621.1; -; mRNA.
DR EMBL; AC004260; AAC34346.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35953.1; -; Genomic_DNA.
DR PIR; T00453; T00453.
DR RefSeq; NP_177848.1; NM_106373.2.
DR AlphaFoldDB; O80658; -.
DR SMR; O80658; -.
DR STRING; 3702.AT1G77240.1; -.
DR PaxDb; O80658; -.
DR PRIDE; O80658; -.
DR ProteomicsDB; 245091; -.
DR EnsemblPlants; AT1G77240.1; AT1G77240.1; AT1G77240.
DR GeneID; 844060; -.
DR Gramene; AT1G77240.1; AT1G77240.1; AT1G77240.
DR KEGG; ath:AT1G77240; -.
DR Araport; AT1G77240; -.
DR TAIR; locus:2195950; AT1G77240.
DR eggNOG; KOG1176; Eukaryota.
DR HOGENOM; CLU_000022_59_5_1; -.
DR InParanoid; O80658; -.
DR OMA; EDGDPKF; -.
DR OrthoDB; 312083at2759; -.
DR PhylomeDB; O80658; -.
DR PRO; PR:O80658; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O80658; differential.
DR Genevisible; O80658; AT.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0006631; P:fatty acid metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.50.12780; -; 1.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig.
DR InterPro; IPR042099; ANL_N_sf.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF13193; AMP-binding_C; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
PE 2: Evidence at transcript level;
KW Fatty acid metabolism; Ligase; Lipid metabolism; Reference proteome.
FT CHAIN 1..545
FT /note="Probable acyl-activating enzyme 4"
FT /id="PRO_0000415715"
FT CONFLICT 123
FT /note="S -> L (in Ref. 1; AAM28621)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 545 AA; 60011 MW; 22CF3108903E6669 CRC64;
MELLLPHASN SCPLTVLGFL ERAASVFGDS PSLLHTTTVH TWSETHSRCL RIASTLSSAS
LGINRGQVVS VIGPNVPSVY ELQFAVPMSG AVLNNINPRL DAHALSVLLR HSESKLVFVD
HHSSSLVLEA VSFLPKDERP RLVILNDGND MPSSSSADMD FLDTYEGFME RGDLRFKWVR
PKSEWTPMVL NYTSGTTSSP KGVVHSHRSV FMSTINSLLD WSLPNRPVYL WTLPMFHANG
WSYTWATAAV GARNICVTRV DVPTIFNLID KYQVTHMCAA PMVLNMLTNH PAQKPLQSPV
KVMTAGAPPP ATVISKAEAL GFDVSHGYGM TETGGLVVSC ALKPEWDRLE PDERAKQKSR
QGIRTAVFAE VDVRDPISGK SVKHDGATVG EIVFRGGSVM LGYYKDPEGT AASMREDGWF
YTGDIGVMHP DGYLEVKDRS KDVVICGGEN ISSTELEAVL YTNPAIKEAA VVAKPDKMWG
ETPCAFVSLK YHDGSVTERE IREFCKTKLP KYMVPRNVVF LEELPKTSTG KIQKFLLRQM
AKSLP
