ID   AAEA_ECO57              Reviewed;         309 AA.
AC   Q8X9E5; Q7AAG0;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=p-hydroxybenzoic acid efflux pump subunit AaeA {ECO:0000255|HAMAP-Rule:MF_01544};
DE            Short=pHBA efflux pump protein A {ECO:0000255|HAMAP-Rule:MF_01544};
GN   Name=aaeA {ECO:0000255|HAMAP-Rule:MF_01544};
GN   OrderedLocusNames=Z4600, ECs4114;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Forms an efflux pump with AaeB. {ECO:0000255|HAMAP-
CC       Rule:MF_01544}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01544}; Single-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01544}.
CC   -!- INDUCTION: Positively coregulated with aaeB and aaeX by AaeR.
CC       {ECO:0000255|HAMAP-Rule:MF_01544}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000255|HAMAP-Rule:MF_01544}.
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DR   EMBL; AE005174; AAG58369.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37537.1; -; Genomic_DNA.
DR   PIR; B91143; B91143.
DR   PIR; E85988; E85988.
DR   RefSeq; NP_312141.1; NC_002695.1.
DR   RefSeq; WP_000854038.1; NZ_SDVX01000004.1.
DR   AlphaFoldDB; Q8X9E5; -.
DR   SMR; Q8X9E5; -.
DR   STRING; 155864.EDL933_4463; -.
DR   EnsemblBacteria; AAG58369; AAG58369; Z4600.
DR   EnsemblBacteria; BAB37537; BAB37537; ECs_4114.
DR   GeneID; 916117; -.
DR   KEGG; ece:Z4600; -.
DR   KEGG; ecs:ECs_4114; -.
DR   PATRIC; fig|386585.9.peg.4295; -.
DR   eggNOG; COG1566; Bacteria.
DR   HOGENOM; CLU_018816_15_2_6; -.
DR   OMA; EVEPTFN; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01544; AaeA; 1.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR022871; PHBA_efflux_pump_AaeA.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..309
FT                   /note="p-hydroxybenzoic acid efflux pump subunit AaeA"
FT                   /id="PRO_0000201851"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01544"
SQ   SEQUENCE   309 AA;  34645 MW;  50A28BC72FB5DA07 CRC64;
     MKTLIRKFSR TAITVVLVIL AFIAIFNAWV YYTESPWTRD ARFSADVVAI APDVSGLITQ
     VNVHNQLVKK GQVLFTIDQP RYQKALEEAQ ADVAYYQVLA QEKRQEAGRR NRLGVQAMSR
     EEIDQANNVL QTVLHQLAKA QATRDLAKLD LERTVIRAPA DGWVTNLNVY TGEFITRGST
     AVALVKQNSF YVLAYMEETK LEGVRPGYRA EITPLGSNKV LKGTVDSVAA GVTNASSTRD
     DKGMATIDSN LEWVRLAQRV PVRIRLDNQQ ENIWPAGTTA TVVVTGKQDR DESQDSFFRK
     MAHRLREFG