PURA_RAT
ID PURA_RAT Reviewed; 138 AA.
AC P86252;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Transcriptional activator protein Pur-alpha {ECO:0000250|UniProtKB:P42669};
DE AltName: Full=Purine-rich single-stranded DNA-binding protein alpha {ECO:0000250|UniProtKB:P42669};
DE Flags: Fragments;
GN Name=Pura {ECO:0000250|UniProtKB:P42669};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE.
RC STRAIN=Wistar; TISSUE=Cerebellum;
RA Maurya D.K., Bhargava P.;
RL Submitted (MAR-2009) to UniProtKB.
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-70, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: This is a probable transcription activator that specifically
CC binds the purine-rich single strand of the PUR element located upstream
CC of the c-Myc gene. May play a role in the initiation of DNA replication
CC and in recombination (By similarity). {ECO:0000250|UniProtKB:P42669}.
CC -!- SUBUNIT: Homodimer, heterodimer with PURB and heterotrimer with PURB
CC and YBX1/Y-box protein 1. Interacts with FMR1; this interaction occurs
CC in association with polyribosome. {ECO:0000250|UniProtKB:P42669}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P42669}.
CC -!- SIMILARITY: Belongs to the PUR DNA-binding protein family.
CC {ECO:0000255}.
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DR AlphaFoldDB; P86252; -.
DR SMR; P86252; -.
DR IntAct; P86252; 3.
DR MINT; P86252; -.
DR iPTMnet; P86252; -.
DR PhosphoSitePlus; P86252; -.
DR SwissPalm; P86252; -.
DR jPOST; P86252; -.
DR PRIDE; P86252; -.
DR UCSC; RGD:1308543; rat.
DR RGD; 1308543; Pura.
DR InParanoid; P86252; -.
DR PhylomeDB; P86252; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0030425; C:dendrite; ISO:RGD.
DR GO; GO:0032839; C:dendrite cytoplasm; IEA:GOC.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:RGD.
DR GO; GO:0043025; C:neuronal cell body; ISO:RGD.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0098794; C:postsynapse; ISO:RGD.
DR GO; GO:0014069; C:postsynaptic density; IDA:SynGO.
DR GO; GO:0003677; F:DNA binding; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; ISO:RGD.
DR GO; GO:0003690; F:double-stranded DNA binding; ISO:RGD.
DR GO; GO:0003691; F:double-stranded telomeric DNA binding; ISO:RGD.
DR GO; GO:0000900; F:mRNA regulatory element binding translation repressor activity; ISO:RGD.
DR GO; GO:0032422; F:purine-rich negative regulatory element binding; ISO:RGD.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; ISO:RGD.
DR GO; GO:0046332; F:SMAD binding; ISO:RGD.
DR GO; GO:0140416; F:transcription regulator inhibitor activity; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; IEA:InterPro.
DR GO; GO:0098963; P:dendritic transport of messenger ribonucleoprotein complex; ISO:RGD.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; ISO:RGD.
DR GO; GO:0050673; P:epithelial cell proliferation; ISO:RGD.
DR GO; GO:0046651; P:lymphocyte proliferation; ISO:RGD.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0007399; P:nervous system development; ISO:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR006628; PUR-bd_fam.
DR InterPro; IPR030500; PURalpha.
DR PANTHER; PTHR12611; PTHR12611; 1.
DR PANTHER; PTHR12611:SF2; PTHR12611:SF2; 1.
DR Pfam; PF04845; PurA; 1.
PE 1: Evidence at protein level;
KW Activator; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN <1..>138
FT /note="Transcriptional activator protein Pur-alpha"
FT /id="PRO_0000371243"
FT MOD_RES 70
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT NON_CONS 13..14
FT /evidence="ECO:0000305"
FT NON_CONS 54..55
FT /evidence="ECO:0000305"
FT NON_CONS 64..65
FT /evidence="ECO:0000305"
FT NON_CONS 87..88
FT /evidence="ECO:0000305"
FT NON_CONS 122..123
FT /evidence="ECO:0000305"
FT NON_CONS 131..132
FT /evidence="ECO:0000305"
FT NON_TER 1
FT NON_TER 138
SQ SEQUENCE 138 AA; 15322 MW; 43C46E1C22B5645B CRC64;
FLKIAEVGAG GNKLTLSMSV AVEFRDYLGD FIEHYAQLGP SQPPDLAQAQ DEPRYYMDLK
ENQRGPGLGS TQGQTIALPA QGLIEFRLID DYGVEEEPAE LPEGTSLTVD NKRFFFDVGS
NKVSEVKPTY RFGHTFCK
