PURE_BRUME
ID PURE_BRUME Reviewed; 162 AA.
AC P52558;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=N5-carboxyaminoimidazole ribonucleotide mutase {ECO:0000255|HAMAP-Rule:MF_01929};
DE Short=N5-CAIR mutase {ECO:0000255|HAMAP-Rule:MF_01929};
DE EC=5.4.99.18 {ECO:0000255|HAMAP-Rule:MF_01929};
DE AltName: Full=5-(carboxyamino)imidazole ribonucleotide mutase {ECO:0000255|HAMAP-Rule:MF_01929};
GN Name=purE {ECO:0000255|HAMAP-Rule:MF_01929}; OrderedLocusNames=BMEI0296;
OS Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=224914;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RA Warren R., Hoover D., Hadfield T., Drazek S.;
RT "Molecular cloning and genetic characterization of the purEK operon of
RT Brucella melitensis strain 16M.";
RL Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=11756688; DOI=10.1073/pnas.221575398;
RA DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA Haselkorn R., Kyrpides N.C., Overbeek R.;
RT "The genome sequence of the facultative intracellular pathogen Brucella
RT melitensis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC -!- FUNCTION: Catalyzes the conversion of N5-carboxyaminoimidazole
CC ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide
CC (CAIR). {ECO:0000255|HAMAP-Rule:MF_01929}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate;
CC Xref=Rhea:RHEA:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:58730,
CC ChEBI:CHEBI:77657; EC=5.4.99.18; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01929};
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-
CC phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.
CC {ECO:0000255|HAMAP-Rule:MF_01929}.
CC -!- SIMILARITY: Belongs to the AIR carboxylase family. Class I subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01929}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL51477.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U10241; AAA57002.1; -; Unassigned_DNA.
DR EMBL; AE008917; AAL51477.1; ALT_INIT; Genomic_DNA.
DR PIR; AB3289; AB3289.
DR RefSeq; WP_002964830.1; NZ_GG703781.1.
DR AlphaFoldDB; P52558; -.
DR SMR; P52558; -.
DR STRING; 224914.BMEI0296; -.
DR EnsemblBacteria; AAL51477; AAL51477; BMEI0296.
DR GeneID; 45125041; -.
DR KEGG; bme:BMEI0296; -.
DR PATRIC; fig|224914.52.peg.1201; -.
DR eggNOG; COG0041; Bacteria.
DR OMA; SNSIDGW; -.
DR PhylomeDB; P52558; -.
DR UniPathway; UPA00074; UER00943.
DR PRO; PR:P52558; -.
DR Proteomes; UP000000419; Chromosome I.
DR GO; GO:0034023; F:5-(carboxyamino)imidazole ribonucleotide mutase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01929; PurE_classI; 1.
DR InterPro; IPR033747; PurE_ClassI.
DR InterPro; IPR000031; PurE_dom.
DR InterPro; IPR024694; PurE_prokaryotes.
DR Pfam; PF00731; AIRC; 1.
DR PIRSF; PIRSF001338; AIR_carboxylase; 1.
DR SMART; SM01001; AIRC; 1.
DR TIGRFAMs; TIGR01162; purE; 1.
PE 3: Inferred from homology;
KW Isomerase; Purine biosynthesis.
FT CHAIN 1..162
FT /note="N5-carboxyaminoimidazole ribonucleotide mutase"
FT /id="PRO_0000074970"
FT BINDING 11
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01929"
FT BINDING 14
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01929"
FT BINDING 41
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01929"
FT CONFLICT 55
FT /note="A -> R (in Ref. 1; AAA57002)"
FT /evidence="ECO:0000305"
FT CONFLICT 67..68
FT /note="GA -> R (in Ref. 1; AAA57002)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 162 AA; 16596 MW; 0B812230C4EE9EE7 CRC64;
MSVDVAIIMG SQSDWETMHH AADTLEALGI SFDARIVSAH RTPDRLVAFA KGAKAEGFKV
IIAGAGGAAH LPGMAAAMTP LPVFGVPVQS KALSGQDSLL SIVQMPAGIP VGTLAIGRAG
AVNAALLAAA VLALYDEALA ARLDEWRKAQ TESVAERPSN EA
