PURE_MYCTU
ID PURE_MYCTU Reviewed; 174 AA.
AC P9WHM1; L0TF53; P96880;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 43.
DE RecName: Full=N5-carboxyaminoimidazole ribonucleotide mutase {ECO:0000255|HAMAP-Rule:MF_01929};
DE Short=N5-CAIR mutase {ECO:0000255|HAMAP-Rule:MF_01929};
DE EC=5.4.99.18 {ECO:0000255|HAMAP-Rule:MF_01929};
DE AltName: Full=5-(carboxyamino)imidazole ribonucleotide mutase {ECO:0000255|HAMAP-Rule:MF_01929};
GN Name=purE {ECO:0000255|HAMAP-Rule:MF_01929}; OrderedLocusNames=Rv3275c;
GN ORFNames=MTCY71.15c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
RX PubMed=19099550; DOI=10.1186/1752-0509-2-109;
RA Raman K., Yeturu K., Chandra N.;
RT "targetTB: a target identification pipeline for Mycobacterium tuberculosis
RT through an interactome, reactome and genome-scale structural analysis.";
RL BMC Syst. Biol. 2:109-109(2008).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Catalyzes the conversion of N5-carboxyaminoimidazole
CC ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide
CC (CAIR). {ECO:0000255|HAMAP-Rule:MF_01929}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate;
CC Xref=Rhea:RHEA:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:58730,
CC ChEBI:CHEBI:77657; EC=5.4.99.18; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01929};
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-
CC phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.
CC {ECO:0000255|HAMAP-Rule:MF_01929}.
CC -!- MISCELLANEOUS: Was identified as a high-confidence drug target.
CC -!- SIMILARITY: Belongs to the AIR carboxylase family. Class I subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_01929}.
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DR EMBL; AL123456; CCP46094.1; -; Genomic_DNA.
DR PIR; D70979; D70979.
DR RefSeq; NP_217792.1; NC_000962.3.
DR RefSeq; WP_003899997.1; NZ_NVQJ01000003.1.
DR PDB; 3LP6; X-ray; 1.70 A; A/B/C/D=1-174.
DR PDBsum; 3LP6; -.
DR AlphaFoldDB; P9WHM1; -.
DR SMR; P9WHM1; -.
DR STRING; 83332.Rv3275c; -.
DR PaxDb; P9WHM1; -.
DR GeneID; 888721; -.
DR KEGG; mtu:Rv3275c; -.
DR TubercuList; Rv3275c; -.
DR eggNOG; COG0041; Bacteria.
DR OMA; SNSIDGW; -.
DR PhylomeDB; P9WHM1; -.
DR UniPathway; UPA00074; UER00943.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0034023; F:5-(carboxyamino)imidazole ribonucleotide mutase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01929; PurE_classI; 1.
DR InterPro; IPR033747; PurE_ClassI.
DR InterPro; IPR000031; PurE_dom.
DR InterPro; IPR024694; PurE_prokaryotes.
DR Pfam; PF00731; AIRC; 1.
DR PIRSF; PIRSF001338; AIR_carboxylase; 1.
DR SMART; SM01001; AIRC; 1.
DR TIGRFAMs; TIGR01162; purE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isomerase; Purine biosynthesis; Reference proteome.
FT CHAIN 1..174
FT /note="N5-carboxyaminoimidazole ribonucleotide mutase"
FT /id="PRO_0000074977"
FT BINDING 16
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01929"
FT BINDING 19
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01929"
FT BINDING 46
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01929"
FT STRAND 9..15
FT /evidence="ECO:0007829|PDB:3LP6"
FT HELIX 17..19
FT /evidence="ECO:0007829|PDB:3LP6"
FT HELIX 20..32
FT /evidence="ECO:0007829|PDB:3LP6"
FT STRAND 37..41
FT /evidence="ECO:0007829|PDB:3LP6"
FT TURN 44..46
FT /evidence="ECO:0007829|PDB:3LP6"
FT HELIX 48..61
FT /evidence="ECO:0007829|PDB:3LP6"
FT STRAND 65..73
FT /evidence="ECO:0007829|PDB:3LP6"
FT HELIX 76..83
FT /evidence="ECO:0007829|PDB:3LP6"
FT STRAND 88..93
FT /evidence="ECO:0007829|PDB:3LP6"
FT HELIX 98..100
FT /evidence="ECO:0007829|PDB:3LP6"
FT HELIX 101..108
FT /evidence="ECO:0007829|PDB:3LP6"
FT HELIX 124..136
FT /evidence="ECO:0007829|PDB:3LP6"
FT HELIX 140..167
FT /evidence="ECO:0007829|PDB:3LP6"
SQ SEQUENCE 174 AA; 17675 MW; 18D5F357FA7BC8D1 CRC64;
MTPAGERPRV GVIMGSDSDW PVMADAAAAL AEFDIPAEVR VVSAHRTPEA MFSYARGAAE
RGLEVIIAGA GGAAHLPGMV AAATPLPVIG VPVPLGRLDG LDSLLSIVQM PAGVPVATVS
IGGAGNAGLL AVRMLGAANP QLRARIVAFQ DRLADVVAAK DAELQRLAGK LTRD
