PURG_MOUSE
ID PURG_MOUSE Reviewed; 350 AA.
AC Q8R4E6; Q14B11; Q8R4E7;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Purine-rich element-binding protein gamma;
GN Name=Purg;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RC STRAIN=B6D2 x J F1, and C57BL/6J; TISSUE=Testis;
RX PubMed=12034829; DOI=10.1093/nar/30.11.2417;
RA Liu H., Johnson E.M.;
RT "Distinct proteins encoded by alternative transcripts of the PURG gene,
RT located contrapodal to WRN on chromosome 8, determined by differential
RT termination/polyadenylation.";
RL Nucleic Acids Res. 30:2417-2426(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-163; SER-166 AND SER-342, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=PURG-A;
CC IsoId=Q8R4E6-1; Sequence=Displayed;
CC Name=2; Synonyms=PURG-A;
CC IsoId=Q8R4E6-2; Sequence=VSP_021320;
CC -!- TISSUE SPECIFICITY: Isoform 1 is expressed in testis. Isoform 2 is
CC expressed in blastocyst and kidney. {ECO:0000269|PubMed:12034829}.
CC -!- SIMILARITY: Belongs to the PUR DNA-binding protein family.
CC {ECO:0000305}.
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DR EMBL; AF479672; AAL86450.1; -; mRNA.
DR EMBL; AF479673; AAL86451.1; -; mRNA.
DR EMBL; BC116406; AAI16407.1; -; mRNA.
DR CCDS; CCDS22230.1; -. [Q8R4E6-1]
DR CCDS; CCDS52538.1; -. [Q8R4E6-2]
DR RefSeq; NP_001091703.1; NM_001098233.1. [Q8R4E6-2]
DR RefSeq; NP_690034.1; NM_152821.2. [Q8R4E6-1]
DR RefSeq; XP_006509269.1; XM_006509206.1.
DR RefSeq; XP_006509272.1; XM_006509209.3. [Q8R4E6-2]
DR AlphaFoldDB; Q8R4E6; -.
DR SMR; Q8R4E6; -.
DR BioGRID; 217164; 1.
DR STRING; 10090.ENSMUSP00000065002; -.
DR iPTMnet; Q8R4E6; -.
DR PhosphoSitePlus; Q8R4E6; -.
DR SwissPalm; Q8R4E6; -.
DR MaxQB; Q8R4E6; -.
DR PaxDb; Q8R4E6; -.
DR PeptideAtlas; Q8R4E6; -.
DR PRIDE; Q8R4E6; -.
DR ProteomicsDB; 300279; -. [Q8R4E6-1]
DR ProteomicsDB; 300280; -. [Q8R4E6-2]
DR Antibodypedia; 23258; 94 antibodies from 16 providers.
DR DNASU; 75029; -.
DR Ensembl; ENSMUST00000070340; ENSMUSP00000065002; ENSMUSG00000049184. [Q8R4E6-1]
DR Ensembl; ENSMUST00000078058; ENSMUSP00000077205; ENSMUSG00000049184. [Q8R4E6-2]
DR GeneID; 75029; -.
DR KEGG; mmu:75029; -.
DR UCSC; uc009ljx.1; mouse. [Q8R4E6-1]
DR UCSC; uc009ljy.1; mouse. [Q8R4E6-2]
DR CTD; 29942; -.
DR MGI; MGI:1922279; Purg.
DR VEuPathDB; HostDB:ENSMUSG00000049184; -.
DR eggNOG; KOG3074; Eukaryota.
DR GeneTree; ENSGT00950000183162; -.
DR HOGENOM; CLU_057873_1_1_1; -.
DR InParanoid; Q8R4E6; -.
DR OMA; MADGCCR; -.
DR OrthoDB; 1248813at2759; -.
DR PhylomeDB; Q8R4E6; -.
DR TreeFam; TF313701; -.
DR BioGRID-ORCS; 75029; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Purg; mouse.
DR PRO; PR:Q8R4E6; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8R4E6; protein.
DR Bgee; ENSMUSG00000049184; Expressed in barrel cortex and 243 other tissues.
DR Genevisible; Q8R4E6; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0032422; F:purine-rich negative regulatory element binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR006628; PUR-bd_fam.
DR InterPro; IPR030501; PURgamma.
DR PANTHER; PTHR12611; PTHR12611; 1.
DR PANTHER; PTHR12611:SF3; PTHR12611:SF3; 1.
DR Pfam; PF04845; PurA; 1.
DR SMART; SM00712; PUR; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..350
FT /note="Purine-rich element-binding protein gamma"
FT /id="PRO_0000255953"
FT DNA_BIND 54..296
FT /evidence="ECO:0000250"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..59
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 136..153
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 163
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 166
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 342
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 292..350
FT /note="VSEVRPPYRNTITVPFKAWTRFGENFIKYEEEMRKICNSHKEKRMDGRRASG
FT EEQECLD -> LTNYPKSRENLNLFHCCQTKHKEQPYDTPKTVEE (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:12034829"
FT /id="VSP_021320"
FT CONFLICT 222
FT /note="D -> E (in Ref. 2; AAI16407)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 350 AA; 39938 MW; 983142173937D7D4 CRC64;
MERARRRGGG GSGGGRGRGG KNVGGPGLSK SRLYPQAQHS HYPHYSASAT PNQSGGTSEI
QELASKRVDI QKKRFYLDVK QSSRGRFLKI AEVWIGRGRQ DNIRKSKLTL SLSVAAELKD
CLGDFIEHYA HLGLKGHRQE HGQSKEQVSR RRQKHSAPSP PVSVGSEEHP HSVLKTDYIE
RDNRKYYLDL KENQRGRFLR IRQTMMRGTG MIGYFGHSLG QDQTIVLPAQ GMIEFRDALV
QLIEDYGEGD IEERRCGDDD PLELPEGTSF RVDNKRFYFD VGSNKYGIFL KVSEVRPPYR
NTITVPFKAW TRFGENFIKY EEEMRKICNS HKEKRMDGRR ASGEEQECLD
