ID   AAEA_ECOLI              Reviewed;         310 AA.
AC   P46482; Q2M8X2;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 153.
DE   RecName: Full=p-hydroxybenzoic acid efflux pump subunit AaeA {ECO:0000255|HAMAP-Rule:MF_01544};
DE            Short=pHBA efflux pump protein A {ECO:0000255|HAMAP-Rule:MF_01544};
GN   Name=aaeA {ECO:0000255|HAMAP-Rule:MF_01544}; Synonyms=yhcQ;
GN   OrderedLocusNames=b3241, JW3210;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   INDUCTION, AND FUNCTION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15489430; DOI=10.1128/jb.186.21.7196-7204.2004;
RA   Van Dyk T.K., Templeton L.J., Cantera K.A., Sharpe P.L., Sariaslani F.S.;
RT   "Characterization of the Escherichia coli AaeAB efflux pump: a metabolic
RT   relief valve?";
RL   J. Bacteriol. 186:7196-7204(2004).
CC   -!- FUNCTION: Forms an efflux pump with AaeB. {ECO:0000255|HAMAP-
CC       Rule:MF_01544, ECO:0000269|PubMed:15489430}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01544}; Single-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01544}.
CC   -!- INDUCTION: Positively coregulated with aaeB and aaeX by AaeR.
CC       {ECO:0000255|HAMAP-Rule:MF_01544, ECO:0000269|PubMed:15489430}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000255|HAMAP-Rule:MF_01544}.
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DR   EMBL; U18997; AAA58043.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76273.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77284.1; -; Genomic_DNA.
DR   PIR; C65116; C65116.
DR   RefSeq; NP_417708.1; NC_000913.3.
DR   RefSeq; WP_000854021.1; NZ_STEB01000012.1.
DR   AlphaFoldDB; P46482; -.
DR   SMR; P46482; -.
DR   BioGRID; 4262447; 215.
DR   DIP; DIP-12289N; -.
DR   IntAct; P46482; 2.
DR   STRING; 511145.b3241; -.
DR   TCDB; 8.A.1.7.1; the membrane fusion protein (mfp) family.
DR   PaxDb; P46482; -.
DR   PRIDE; P46482; -.
DR   EnsemblBacteria; AAC76273; AAC76273; b3241.
DR   EnsemblBacteria; BAE77284; BAE77284; BAE77284.
DR   GeneID; 66672863; -.
DR   GeneID; 947748; -.
DR   KEGG; ecj:JW3210; -.
DR   KEGG; eco:b3241; -.
DR   PATRIC; fig|1411691.4.peg.3487; -.
DR   EchoBASE; EB2674; -.
DR   eggNOG; COG1566; Bacteria.
DR   HOGENOM; CLU_018816_15_2_6; -.
DR   InParanoid; P46482; -.
DR   OMA; EVEPTFN; -.
DR   PhylomeDB; P46482; -.
DR   BioCyc; EcoCyc:G7686-MON; -.
DR   BioCyc; MetaCyc:G7686-MON; -.
DR   PRO; PR:P46482; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046942; P:carboxylic acid transport; IGI:EcoliWiki.
DR   HAMAP; MF_01544; AaeA; 1.
DR   InterPro; IPR039562; MFP_biotin_lipoyl_2.
DR   InterPro; IPR022871; PHBA_efflux_pump_AaeA.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF13533; Biotin_lipoyl_2; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..310
FT                   /note="p-hydroxybenzoic acid efflux pump subunit AaeA"
FT                   /id="PRO_0000201850"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01544"
SQ   SEQUENCE   310 AA;  34775 MW;  6E0232D459DC2757 CRC64;
     MKTLIRKFSR TAITVVLVIL AFIAIFNAWV YYTESPWTRD ARFSADVVAI APDVSGLITQ
     VNVHDNQLVK KGQILFTIDQ PRYQKALEEA QADVAYYQVL AQEKRQEAGR RNRLGVQAMS
     REEIDQANNV LQTVLHQLAK AQATRDLAKL DLERTVIRAP ADGWVTNLNV YTGEFITRGS
     TAVALVKQNS FYVLAYMEET KLEGVRPGYR AEITPLGSNK VLKGTVDSVA AGVTNASSTR
     DDKGMATIDS NLEWVRLAQR VPVRIRLDNQ QENIWPAGTT ATVVVTGKQD RDESQDSFFR
     KMAHRLREFG