PURU_CORS1
ID PURU_CORS1 Reviewed; 286 AA.
AC Q46339;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Formyltetrahydrofolate deformylase {ECO:0000255|HAMAP-Rule:MF_01927};
DE EC=3.5.1.10 {ECO:0000255|HAMAP-Rule:MF_01927};
DE AltName: Full=Formyl-FH(4) hydrolase {ECO:0000255|HAMAP-Rule:MF_01927};
GN Name=purU {ECO:0000255|HAMAP-Rule:MF_01927};
OS Corynebacterium sp. (strain P-1).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium; unclassified Corynebacterium.
OX NCBI_TaxID=69006;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7543100; DOI=10.1074/jbc.270.31.18252;
RA Chlumsky L.J., Zhang L., Jorns M.S.;
RT "Sequence analysis of sarcosine oxidase and nearby genes reveals homologies
RT with key enzymes of folate one-carbon metabolism.";
RL J. Biol. Chem. 270:18252-18259(1995).
CC -!- FUNCTION: Catalyzes the hydrolysis of 10-formyltetrahydrofolate
CC (formyl-FH4) to formate and tetrahydrofolate (FH4). {ECO:0000255|HAMAP-
CC Rule:MF_01927}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-10-formyltetrahydrofolate + H2O = (6S)-5,6,7,8-
CC tetrahydrofolate + formate + H(+); Xref=Rhea:RHEA:19833,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454; EC=3.5.1.10;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01927};
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.
CC {ECO:0000255|HAMAP-Rule:MF_01927}.
CC -!- SIMILARITY: Belongs to the PurU family. {ECO:0000255|HAMAP-
CC Rule:MF_01927}.
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DR EMBL; U23955; AAC43463.1; -; Genomic_DNA.
DR PIR; I40891; I40891.
DR AlphaFoldDB; Q46339; -.
DR SMR; Q46339; -.
DR UniPathway; UPA00074; UER00170.
DR GO; GO:0008864; F:formyltetrahydrofolate deformylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016742; F:hydroxymethyl-, formyl- and related transferase activity; IEA:InterPro.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR CDD; cd04875; ACT_F4HF-DF; 1.
DR CDD; cd08648; FMT_core_Formyl-FH4-Hydrolase_C; 1.
DR HAMAP; MF_01927; PurU; 1.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR041729; Formyl-FH4-Hydrolase_C.
DR InterPro; IPR002376; Formyl_transf_N.
DR InterPro; IPR036477; Formyl_transf_N_sf.
DR InterPro; IPR004810; PurU.
DR InterPro; IPR044074; PurU_ACT.
DR PANTHER; PTHR42706; PTHR42706; 1.
DR Pfam; PF00551; Formyl_trans_N; 1.
DR PRINTS; PR01575; FFH4HYDRLASE.
DR SUPFAM; SSF53328; SSF53328; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR TIGRFAMs; TIGR00655; PurU; 1.
DR PROSITE; PS51671; ACT; 1.
PE 3: Inferred from homology;
KW Hydrolase; One-carbon metabolism; Purine biosynthesis.
FT CHAIN 1..286
FT /note="Formyltetrahydrofolate deformylase"
FT /id="PRO_0000074960"
FT DOMAIN 8..88
FT /note="ACT"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01927"
FT ACT_SITE 230
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01927"
SQ SEQUENCE 286 AA; 32111 MW; AE094C41C9042426 CRC64;
MSEQVRHVLT LQCPEGIGIV HAVTGFLVRH QRTIVELKQY DDMSAGRLFL RVDFAGDSAP
DLLDALRSEF SEVAAKFDMD WQLRERGQKT KVLIMVSKFE HCLQDLLFRM HSGDLPIEVV
GVASNHPDHR SLVEWYGIGF HHIPISKDTK PRAEAALLEL IDQTGAELVV LARYMQVLSD
HLASELTGKT INIHHSFLPS FKGAKPYHQA WERGVKTVGA TAHYVNSELD EGPIIAQQVV
EVDHTYGPQD LVAAGRDSEC KALSNAVRWH CEGRVFLYGN RTVVLR
