PURU_HAEIN
ID PURU_HAEIN Reviewed; 278 AA.
AC Q03432;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Formyltetrahydrofolate deformylase {ECO:0000255|HAMAP-Rule:MF_01927};
DE EC=3.5.1.10 {ECO:0000255|HAMAP-Rule:MF_01927};
DE AltName: Full=Formyl-FH(4) hydrolase {ECO:0000255|HAMAP-Rule:MF_01927};
GN Name=purU {ECO:0000255|HAMAP-Rule:MF_01927}; OrderedLocusNames=HI_1588;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 64-278.
RC STRAIN=RM 7004 / Serotype B;
RX PubMed=8335255; DOI=10.1016/0378-1119(93)90713-d;
RA Maskell D.J.;
RT "Cloning and sequencing of the Haemophilus influenzae aroA gene.";
RL Gene 129:155-156(1993).
CC -!- FUNCTION: Catalyzes the hydrolysis of 10-formyltetrahydrofolate
CC (formyl-FH4) to formate and tetrahydrofolate (FH4). {ECO:0000255|HAMAP-
CC Rule:MF_01927}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-10-formyltetrahydrofolate + H2O = (6S)-5,6,7,8-
CC tetrahydrofolate + formate + H(+); Xref=Rhea:RHEA:19833,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454; EC=3.5.1.10;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01927};
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.
CC {ECO:0000255|HAMAP-Rule:MF_01927}.
CC -!- SIMILARITY: Belongs to the PurU family. {ECO:0000255|HAMAP-
CC Rule:MF_01927}.
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DR EMBL; L42023; AAC23236.1; -; Genomic_DNA.
DR EMBL; L04686; AAA24942.1; -; Genomic_DNA.
DR PIR; E64131; E64131.
DR RefSeq; NP_439733.1; NC_000907.1.
DR RefSeq; WP_005693604.1; NC_000907.1.
DR AlphaFoldDB; Q03432; -.
DR SMR; Q03432; -.
DR STRING; 71421.HI_1588; -.
DR PRIDE; Q03432; -.
DR EnsemblBacteria; AAC23236; AAC23236; HI_1588.
DR KEGG; hin:HI_1588; -.
DR PATRIC; fig|71421.8.peg.1662; -.
DR eggNOG; COG0788; Bacteria.
DR HOGENOM; CLU_038395_3_2_6; -.
DR OMA; HADHHTD; -.
DR PhylomeDB; Q03432; -.
DR BioCyc; HINF71421:G1GJ1-1604-MON; -.
DR UniPathway; UPA00074; UER00170.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0008864; F:formyltetrahydrofolate deformylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016742; F:hydroxymethyl-, formyl- and related transferase activity; IEA:InterPro.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR CDD; cd04875; ACT_F4HF-DF; 1.
DR CDD; cd08648; FMT_core_Formyl-FH4-Hydrolase_C; 1.
DR HAMAP; MF_01927; PurU; 1.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR041729; Formyl-FH4-Hydrolase_C.
DR InterPro; IPR002376; Formyl_transf_N.
DR InterPro; IPR036477; Formyl_transf_N_sf.
DR InterPro; IPR004810; PurU.
DR InterPro; IPR044074; PurU_ACT.
DR PANTHER; PTHR42706; PTHR42706; 1.
DR Pfam; PF01842; ACT; 1.
DR Pfam; PF00551; Formyl_trans_N; 1.
DR PRINTS; PR01575; FFH4HYDRLASE.
DR SUPFAM; SSF53328; SSF53328; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR TIGRFAMs; TIGR00655; PurU; 1.
DR PROSITE; PS51671; ACT; 1.
PE 3: Inferred from homology;
KW Hydrolase; One-carbon metabolism; Purine biosynthesis; Reference proteome.
FT CHAIN 1..278
FT /note="Formyltetrahydrofolate deformylase"
FT /id="PRO_0000074963"
FT DOMAIN 6..85
FT /note="ACT"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01927"
FT ACT_SITE 223
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01927"
FT CONFLICT 115..117
FT /note="VIG -> RNR (in Ref. 2; AAA24942)"
FT /evidence="ECO:0000305"
FT CONFLICT 138..140
FT /note="HEN -> PK (in Ref. 2; AAA24942)"
FT /evidence="ECO:0000305"
FT CONFLICT 205
FT /note="K -> E (in Ref. 2; AAA24942)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 278 AA; 32173 MW; 7F375AB3C422EC4B CRC64;
MIEKKILLTD CPDDKGLIAK ITNICYKHQL NILHNNEFVD FETKHFFMRT ELEGIFNEAT
LLEDLKYSLP EETNCRLIGT QRKRIVILVT KEAHCLGDIL MKNYYGALDV EIAAVIGNHD
NLRELVERFN IPFHLVSHEN LTRVEHDKLL AEKIDEYTPD YIVLAKYMRV LNPEFVARYP
NRVINIHHSF LPAFIGAKPY QQAYKRGVKI IGATAHFINN ELDQGPIIMQ NVINVDHTYN
AEAMMRAGRD VEKTVLSRAL DLALHDRIFV YKNKTVVL
