PURU_SYNY3
ID PURU_SYNY3 Reviewed; 284 AA.
AC Q55135;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Formyltetrahydrofolate deformylase {ECO:0000255|HAMAP-Rule:MF_01927};
DE EC=3.5.1.10 {ECO:0000255|HAMAP-Rule:MF_01927};
DE AltName: Full=Formyl-FH(4) hydrolase {ECO:0000255|HAMAP-Rule:MF_01927};
GN Name=purU {ECO:0000255|HAMAP-Rule:MF_01927}; OrderedLocusNames=sll0070;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- FUNCTION: Catalyzes the hydrolysis of 10-formyltetrahydrofolate
CC (formyl-FH4) to formate and tetrahydrofolate (FH4). {ECO:0000255|HAMAP-
CC Rule:MF_01927}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-10-formyltetrahydrofolate + H2O = (6S)-5,6,7,8-
CC tetrahydrofolate + formate + H(+); Xref=Rhea:RHEA:19833,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454; EC=3.5.1.10;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01927};
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.
CC {ECO:0000255|HAMAP-Rule:MF_01927}.
CC -!- SIMILARITY: Belongs to the PurU family. {ECO:0000255|HAMAP-
CC Rule:MF_01927}.
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DR EMBL; BA000022; BAA10270.1; -; Genomic_DNA.
DR PIR; S74352; S74352.
DR AlphaFoldDB; Q55135; -.
DR SMR; Q55135; -.
DR IntAct; Q55135; 3.
DR STRING; 1148.1001129; -.
DR PaxDb; Q55135; -.
DR EnsemblBacteria; BAA10270; BAA10270; BAA10270.
DR KEGG; syn:sll0070; -.
DR eggNOG; COG0788; Bacteria.
DR InParanoid; Q55135; -.
DR OMA; HADHHTD; -.
DR PhylomeDB; Q55135; -.
DR UniPathway; UPA00074; UER00170.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0008864; F:formyltetrahydrofolate deformylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016742; F:hydroxymethyl-, formyl- and related transferase activity; IEA:InterPro.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR CDD; cd04875; ACT_F4HF-DF; 1.
DR CDD; cd08648; FMT_core_Formyl-FH4-Hydrolase_C; 1.
DR HAMAP; MF_01927; PurU; 1.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR041729; Formyl-FH4-Hydrolase_C.
DR InterPro; IPR002376; Formyl_transf_N.
DR InterPro; IPR036477; Formyl_transf_N_sf.
DR InterPro; IPR004810; PurU.
DR InterPro; IPR044074; PurU_ACT.
DR PANTHER; PTHR42706; PTHR42706; 1.
DR Pfam; PF01842; ACT; 1.
DR Pfam; PF00551; Formyl_trans_N; 1.
DR PRINTS; PR01575; FFH4HYDRLASE.
DR SUPFAM; SSF53328; SSF53328; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
DR TIGRFAMs; TIGR00655; PurU; 1.
DR PROSITE; PS51671; ACT; 1.
PE 3: Inferred from homology;
KW Hydrolase; One-carbon metabolism; Purine biosynthesis; Reference proteome.
FT CHAIN 1..284
FT /note="Formyltetrahydrofolate deformylase"
FT /id="PRO_0000074967"
FT DOMAIN 7..90
FT /note="ACT"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01927"
FT ACT_SITE 228
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01927"
SQ SEQUENCE 284 AA; 32634 MW; D9C0ADC38AE537C9 CRC64;
MQNLTATLLV SCPDQPGIVA QIAQFIYQNQ GNIIHADQHT DFSSGLFLNR VEWQLDNFRL
SRPELLSAWS QLAEQLQATW QIHFSDQLPR LALWVSKQDH CLLDILWRWR SGELRCEIPL
IISNHPDLKS IADQFGIDFH CLPITKENKL AQETAELALL KQYQIDLVVL AKYLQILTTD
FVVQFPNIIN IHHSFLPAFP GANPYHRAHE RGVKIIGATA HYATAQLDEG PIIEQDVVRV
SHRDNVDDLI RKGRDLERVV LARAVRLHLQ HRILVYDNRT VVFA
