ID   PUS10_ACIS3             Reviewed;         400 AA.
AC   D9PZU4;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01893};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
DE            Short=Psi54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
GN   Name=pus10 {ECO:0000255|HAMAP-Rule:MF_01893}; OrderedLocusNames=ASAC_0174;
OS   Acidilobus saccharovorans (strain DSM 16705 / JCM 18335 / VKM B-2471 /
OS   345-15).
OC   Archaea; Crenarchaeota; Thermoprotei; Acidilobales; Acidilobaceae;
OC   Acidilobus.
OX   NCBI_TaxID=666510;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16705 / JCM 18335 / VKM B-2471 / 345-15;
RX   PubMed=20581186; DOI=10.1128/aem.00599-10;
RA   Mardanov A.V., Svetlitchnyi V.A., Beletsky A.V., Prokofeva M.I.,
RA   Bonch-Osmolovskaya E.A., Ravin N.V., Skryabin K.G.;
RT   "The genome sequence of the crenarchaeon Acidilobus saccharovorans supports
RT   a new order, Acidilobales, and suggests an important ecological role in
RT   terrestrial acidic hot springs.";
RL   Appl. Environ. Microbiol. 76:5652-5657(2010).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01893}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01893};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01893};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01893}.
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DR   EMBL; CP001742; ADL18582.1; -; Genomic_DNA.
DR   RefSeq; WP_013266094.1; NC_014374.1.
DR   AlphaFoldDB; D9PZU4; -.
DR   STRING; 666510.ASAC_0174; -.
DR   EnsemblBacteria; ADL18582; ADL18582; ASAC_0174.
DR   GeneID; 9498390; -.
DR   KEGG; asc:ASAC_0174; -.
DR   eggNOG; arCOG01015; Archaea.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   OMA; DGGLYIK; -.
DR   OrthoDB; 61936at2157; -.
DR   Proteomes; UP000000346; Chromosome.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR004114; THUMP_dom.
DR   Pfam; PF02926; THUMP; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..400
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000407380"
FT   DOMAIN          77..194
FT                   /note="THUMP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         301
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
SQ   SEQUENCE   400 AA;  43128 MW;  32E2010D15DE1C7C CRC64;
     MNEDPAQQAM KVLAKYPLCD RCLGRLFAGL GRGLSNAERG RALKLSVVMS LHAMSLSGRL
     PENARDVLAN AGGAAAQVYV ELFGSPPEHR SCYICNDALD KFLDEMPQRV ADAVKEWGGS
     TFLVGAKVDP GVIAREERIK AEFGLAFGES IKSEIKRELG KRAQQLGPKV SFDRPDVVVM
     VSFPDGSVSV QPRRLVVKGL YRRLRRDLQL RPREALSHPL VARLINDTRS SRVGIVGLVR
     DEKEVRALGL GIPVEFHLGG PRVRLVPPDG SIIEAEGASL EVNSSVEASG PEDLSRRVRV
     YRCVLYVEGG AFESLQLAAA SLRGKEVRQK FMGKEVSGVV RGAECVDMGG GLAECIIALD
     ERLHVMELVS GSGTEPSLSG LLGTAAECVV ADLLGVIPLR