ID   PUS10_CALMQ             Reviewed;         401 AA.
AC   A8MDD0;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01893};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
DE            Short=Psi54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
GN   Name=pus10 {ECO:0000255|HAMAP-Rule:MF_01893}; OrderedLocusNames=Cmaq_0954;
OS   Caldivirga maquilingensis (strain ATCC 700844 / DSM 13496 / JCM 10307 /
OS   IC-167).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Caldivirga.
OX   NCBI_TaxID=397948;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700844 / DSM 13496 / JCM 10307 / IC-167;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Saunders E., Brettin T., Bruce D., Detter J.C.,
RA   Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Ivanova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M.,
RA   Saltikov C., House C.H., Richardson P.;
RT   "Complete sequence of Caldivirga maquilingensis IC-167.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01893}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01893};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01893};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01893}.
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DR   EMBL; CP000852; ABW01786.1; -; Genomic_DNA.
DR   RefSeq; WP_012186005.1; NC_009954.1.
DR   AlphaFoldDB; A8MDD0; -.
DR   SMR; A8MDD0; -.
DR   STRING; 397948.Cmaq_0954; -.
DR   EnsemblBacteria; ABW01786; ABW01786; Cmaq_0954.
DR   GeneID; 5708948; -.
DR   KEGG; cma:Cmaq_0954; -.
DR   eggNOG; arCOG01015; Archaea.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   OMA; DGGLYIK; -.
DR   OrthoDB; 61936at2157; -.
DR   Proteomes; UP000001137; Chromosome.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR039894; Pus10-like.
DR   InterPro; IPR004114; THUMP_dom.
DR   PANTHER; PTHR21568; PTHR21568; 2.
DR   Pfam; PF02926; THUMP; 1.
DR   TIGRFAMs; TIGR01213; pseudo_Pus10arc; 1.
DR   PROSITE; PS51165; THUMP; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..401
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000407383"
FT   DOMAIN          64..195
FT                   /note="THUMP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
SQ   SEQUENCE   401 AA;  44601 MW;  971DC62529E6BAD1 CRC64;
     MSIIEDARKA LLKYPLCDHC LGRLFASRGL MISNEERGRS IKNVLFMESL NSSTGTYNED
     TLVALAKSGH RESLLFLRRM GKVIEQQPCF ICGNLFDKIN INDIVSKVEE EINRQGIEFN
     SFQVGSTVNK GVIENEVKVS TELGITSSES IKRELNRLIG KVLADKLGKR YSRLNPDVVI
     KVNTSDGSVS VEVMPIYIEA RYRKLIRGIP QVGDSSVASV AREVVSELRP LNVVLHFAGI
     EGPEVRVLGL GRPMIIEATR PLRRSLPGGI ITRHGVQLLN LKAAGKVQVR EIKSKAGELR
     RVFRILVKLH GSVTDEQLRS LEDYFSNRQI RQSMGKGRRV KLIYGLKVVP VHGSILELIV
     NTQGGFSVRR FITGEGTEPS VSGTLGIKVT PIEIDILNIW H