ID   PUS10_CENSY             Reviewed;         402 AA.
AC   A0RUP0;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01893};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
DE            Short=Psi54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
GN   Name=pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
GN   OrderedLocusNames=CENSYa_0422;
OS   Cenarchaeum symbiosum (strain A).
OC   Archaea; Thaumarchaeota; Cenarchaeales; Cenarchaeaceae; Cenarchaeum.
OX   NCBI_TaxID=414004;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=A;
RX   PubMed=17114289; DOI=10.1073/pnas.0608549103;
RA   Hallam S.J., Konstantinidis K.T., Putnam N., Schleper C., Watanabe Y.,
RA   Sugahara J., Preston C., de la Torre J., Richardson P.M., DeLong E.F.;
RT   "Genomic analysis of the uncultivated marine crenarchaeote Cenarchaeum
RT   symbiosum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:18296-18301(2006).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01893}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01893};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01893};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01893}.
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DR   EMBL; DP000238; ABK77057.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0RUP0; -.
DR   STRING; 414004.CENSYa_0422; -.
DR   EnsemblBacteria; ABK77057; ABK77057; CENSYa_0422.
DR   KEGG; csy:CENSYa_0422; -.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   Proteomes; UP000000758; Chromosome.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR039894; Pus10-like.
DR   PANTHER; PTHR21568; PTHR21568; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..402
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000407384"
FT   REGION          370..402
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        215
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
SQ   SEQUENCE   402 AA;  42855 MW;  F24A4405F9FC32F6 CRC64;
     MRPERGYVLC DPCLGRLFGS GNILHCEKRG RIIRGKGRDR PSECHICKGL CPSLEESCGP
     SLWGAAAYEF ETFVVGVRLK SSMAERDDEV RSRCRLAGAA ALRAYVAAML SSYLGAMTGA
     SVDHGAPELS ITADLRDNTA EFHPRPVVLS GRYTKSVRGL SQKGAPCGGC AGEGCTSCGM
     LGVDTGGSVE GVISGHACGT YSARRASFTW VGGEDQESLV GGRGRPFVAQ LVQPHRRGLV
     HPATVRLGGV VLHGLREVRS MPQLPVRFRS KVRLAVSAKG EISDDTLGRL ADLGGSTLAV
     YEGRRRVERA IHSASHSRTG PGSFELHMEV DGGVPFKRFV SGETVFPNLS DLLGTVCSCG
     GFDFEEITVE RGGHPGARGG TRRRPRKGPA RPAGGRDRPR KT