PUS10_DROME
ID PUS10_DROME Reviewed; 500 AA.
AC Q9VPK7;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Putative tRNA pseudouridine synthase Pus10;
DE EC=5.4.99.25;
DE AltName: Full=Coiled-coil domain-containing protein 139 homolog;
DE AltName: Full=tRNA pseudouridine 55 synthase;
DE Short=Psi55 synthase;
DE AltName: Full=tRNA pseudouridylate synthase;
DE AltName: Full=tRNA-uridine isomerase;
GN Name=Pus10; ORFNames=CG3709;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-55 in
CC the psi GC loop of transfer RNAs. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC {ECO:0000305}.
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DR EMBL; AE014134; AAF51539.2; -; Genomic_DNA.
DR EMBL; AY069446; AAL39591.1; -; mRNA.
DR RefSeq; NP_608500.1; NM_134656.4.
DR AlphaFoldDB; Q9VPK7; -.
DR SMR; Q9VPK7; -.
DR BioGRID; 59438; 2.
DR IntAct; Q9VPK7; 1.
DR STRING; 7227.FBpp0077806; -.
DR PaxDb; Q9VPK7; -.
DR PRIDE; Q9VPK7; -.
DR DNASU; 33178; -.
DR EnsemblMetazoa; FBtr0078147; FBpp0077806; FBgn0031227.
DR GeneID; 33178; -.
DR KEGG; dme:Dmel_CG3709; -.
DR UCSC; CG3709-RA; d. melanogaster.
DR FlyBase; FBgn0031227; CG3709.
DR VEuPathDB; VectorBase:FBgn0031227; -.
DR eggNOG; KOG2364; Eukaryota.
DR GeneTree; ENSGT00390000007529; -.
DR HOGENOM; CLU_028780_2_0_1; -.
DR InParanoid; Q9VPK7; -.
DR OMA; INIDVRH; -.
DR OrthoDB; 1067006at2759; -.
DR PhylomeDB; Q9VPK7; -.
DR BioGRID-ORCS; 33178; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 33178; -.
DR PRO; PR:Q9VPK7; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031227; Expressed in egg chamber and 25 other tissues.
DR Genevisible; Q9VPK7; DM.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR039894; Pus10-like.
DR PANTHER; PTHR21568; PTHR21568; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..500
FT /note="Putative tRNA pseudouridine synthase Pus10"
FT /id="PRO_0000299025"
FT REGION 267..280
FT /note="RNA binding forefinger loop"
FT /evidence="ECO:0000255"
FT REGION 406..421
FT /note="RNA binding thumb loop"
FT /evidence="ECO:0000255"
FT COILED 30..50
FT /evidence="ECO:0000255"
FT ACT_SITE 309
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
FT BINDING 378
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 451
FT /ligand="substrate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 500 AA; 56763 MW; D0FA2B91035D78F7 CRC64;
MKNQDLVEYL RSCGVCEICQ LRYLKARGAE YRNINETLQK LDVKLNENVE NEELVISEGS
QPSKRARLGV CSTCLGLFSK DFQNELLSSI LASDFAKYDC QKIVLAICLP MSLQLRQLAM
WFALRRRFGP SIDENNPPDV PIKEAVKLIL HPIICEKLAK VYDANGLMIN IDLTHSLEGE
EVEKLVKLNK EAFPAKASHQ KRIEISRGLL EKQYQPSKVK AETYEKYFQI PCSNVEESIK
LTSIDLQGPL ICVAGRYRKL SRELSHTPWI LNGQRLMEDS IEEIIIRHVG PHFTEKLGKI
TFMSSGREDV DVRCLGKGRP FVLEIPNAKR SCLTKDEAFA MERAVDASGM VSIHHLQVVP
REELTHIKTG EEQKKKFYRA LCALQEPVSV KILEQLQISA SFNIQQKTPI RVLHRRPLHT
RPRTIYSVTA KVHRGNPKAL IIDIVTQAGT YIKELVHGEF GRTTPSLSSI IGKPMDIQAL
DVVGIDLDWP HEVDNSKHKE
