ID   PUS10_IGNAA             Reviewed;         430 AA.
AC   E0SNQ8;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01893};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
DE            Short=Psi54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
GN   Name=pus10 {ECO:0000255|HAMAP-Rule:MF_01893}; OrderedLocusNames=Igag_1040;
OS   Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1).
OC   Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC   Desulfurococcaceae; Ignisphaera.
OX   NCBI_TaxID=583356;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17230 / JCM 13409 / AQ1.S1;
RX   PubMed=21304693; DOI=10.4056/sigs.1072907;
RA   Goker M., Held B., Lapidus A., Nolan M., Spring S., Yasawong M., Lucas S.,
RA   Glavina Del Rio T., Tice H., Cheng J.F., Goodwin L., Tapia R., Pitluck S.,
RA   Liolios K., Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA   Palaniappan K., Brambilla E., Land M., Hauser L., Chang Y.J.,
RA   Jeffries C.D., Brettin T., Detter J.C., Han C., Rohde M., Sikorski J.,
RA   Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Ignisphaera aggregans type strain (AQ1.S1).";
RL   Stand. Genomic Sci. 3:66-75(2010).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01893}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01893};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01893};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01893}.
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DR   EMBL; CP002098; ADM27854.1; -; Genomic_DNA.
DR   AlphaFoldDB; E0SNQ8; -.
DR   SMR; E0SNQ8; -.
DR   STRING; 583356.Igag_1040; -.
DR   EnsemblBacteria; ADM27854; ADM27854; Igag_1040.
DR   KEGG; iag:Igag_1040; -.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   OMA; DGGLYIK; -.
DR   Proteomes; UP000001304; Chromosome.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR039894; Pus10-like.
DR   InterPro; IPR004114; THUMP_dom.
DR   PANTHER; PTHR21568; PTHR21568; 1.
DR   Pfam; PF02926; THUMP; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR01213; pseudo_Pus10arc; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..430
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000407386"
FT   ACT_SITE        253
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         320
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         392
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
SQ   SEQUENCE   430 AA;  50020 MW;  D7127DF93B473F90 CRC64;
     MSIIDIANNI LRKYCLCDRC LGRLFASLGR GLSNDERGKA IKIALVLELH KRYLDGDKDS
     LKQLVELSPN IGPIAVNLIK NLGIEIEYTP RTCFICDNKI NDIIDTYSQR IADIINERHI
     NSFVLGIRGV TSYIRKEESI ANEFKLLYWE NIKRELKREI GKKIQMMTNA KVDFLNPEAM
     IIIDIDRDRI YIESPSLLIY GRYWKLGRMI SQNIWLTKNG VKKYPLSIEE IAKMLVKDGF
     GDDVVLHIAG REDVDVRTLG SGRPFVLEIK RPRKRNIDIK DIENRLNSIS RWLKFELNMF
     VDRDFVSRVK KGCRTSYKIY RAIVVADRDI GIEDIKRLEE FFRDRIIEQR TPTRVLRRKK
     DVLRRRKVFE IKTRVISPRV FEALIKCEGG LYVKELISGD NGRTVPSFSS VLNANTLCLT
     LDALYVHEYI