ID   PUS10_METJA             Reviewed;         454 AA.
AC   Q60346;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=tRNA pseudouridine synthase Pus10;
DE            EC=5.4.99.25 {ECO:0000269|PubMed:18952823};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase;
DE            Short=Psi54/55 synthase;
GN   Name=pus10; OrderedLocusNames=MJ0041;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=18952823; DOI=10.1261/rna.1276508;
RA   Gurha P., Gupta R.;
RT   "Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in
RT   tRNA.";
RL   RNA 14:2521-2527(2008).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs.
CC       {ECO:0000269|PubMed:18952823}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000269|PubMed:18952823};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000269|PubMed:18952823};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L77117; AAB98022.1; -; Genomic_DNA.
DR   PIR; A64305; A64305.
DR   AlphaFoldDB; Q60346; -.
DR   SMR; Q60346; -.
DR   STRING; 243232.MJ_0041; -.
DR   EnsemblBacteria; AAB98022; AAB98022; MJ_0041.
DR   KEGG; mja:MJ_0041; -.
DR   eggNOG; arCOG01015; Archaea.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   InParanoid; Q60346; -.
DR   OMA; DGGLYIK; -.
DR   PhylomeDB; Q60346; -.
DR   BRENDA; 5.4.99.25; 3260.
DR   BRENDA; 5.4.99.B22; 3260.
DR   BRENDA; 5.4.99.B25; 3260.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IDA:UniProtKB.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IDA:UniProtKB.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR039894; Pus10-like.
DR   InterPro; IPR004114; THUMP_dom.
DR   PANTHER; PTHR21568; PTHR21568; 2.
DR   Pfam; PF02926; THUMP; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR01213; pseudo_Pus10arc; 1.
DR   PROSITE; PS51165; THUMP; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..454
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000106664"
FT   DOMAIN          100..206
FT                   /note="THUMP"
FT   ACT_SITE        275
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255"
FT   BINDING         339
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
FT   BINDING         411
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   454 AA;  52927 MW;  C91F61339D284209 CRC64;
     MASIINKQII SPTGRNMEII NYEILKKYPL CDRCFGRLYA KLLHTTNTER GRALKLYKAL
     ELEAKIKKAK EKGINYEEEL ELLKALAKSG VDEIRLEDIE IEKENCPWCR GIFNKQKMEK
     LLNKAIELLK EYDFDTFLIG THIPEEIKDL EKEIETEFME SIKQEFGREF GKMLAVRLDK
     APDKEYPDIV VHINPYTEEI YLQINPLFIK GRYRKLVRGI PQTRWPCRKC RGKGCELCNY
     TGKKYPISVE EIIAKPFLEA TKGVDAKFHG AGREDIDVRM LGDGRPFVLE IKEPKIRKID
     LNKIAEEINK DGRVEVLNLE FGVRKDKVIF KNTPHRKTYR ALVECSDKIT DEELKLLEKE
     LENRTIYQKT PKRVLHRRAD LERIRKVYKV KTSKVDDNHF EMIIYCDGGL YIKELISGDD
     GRTNPSVSSI LNKNCICKEL DVLKIHDNNL LEKG