ID   PUS10_METST             Reviewed;         407 AA.
AC   Q2NE45;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01893};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
DE            Short=Psi54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
GN   Name=pus10 {ECO:0000255|HAMAP-Rule:MF_01893}; OrderedLocusNames=Msp_1541;
OS   Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 /
OS   MCB-3).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanosphaera.
OX   NCBI_TaxID=339860;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3;
RX   PubMed=16385054; DOI=10.1128/jb.188.2.642-658.2006;
RA   Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., Hedderich R.,
RA   Gottschalk G., Thauer R.K.;
RT   "The genome sequence of Methanosphaera stadtmanae reveals why this human
RT   intestinal archaeon is restricted to methanol and H2 for methane formation
RT   and ATP synthesis.";
RL   J. Bacteriol. 188:642-658(2006).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01893}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01893};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01893};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01893}.
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DR   EMBL; CP000102; ABC57908.1; -; Genomic_DNA.
DR   RefSeq; WP_011407107.1; NC_007681.1.
DR   AlphaFoldDB; Q2NE45; -.
DR   SMR; Q2NE45; -.
DR   STRING; 339860.Msp_1541; -.
DR   PRIDE; Q2NE45; -.
DR   EnsemblBacteria; ABC57908; ABC57908; Msp_1541.
DR   GeneID; 41326117; -.
DR   KEGG; mst:Msp_1541; -.
DR   eggNOG; arCOG01015; Archaea.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   OMA; DGGLYIK; -.
DR   OrthoDB; 61936at2157; -.
DR   Proteomes; UP000001931; Chromosome.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR039894; Pus10-like.
DR   InterPro; IPR004114; THUMP_dom.
DR   PANTHER; PTHR21568; PTHR21568; 1.
DR   Pfam; PF02926; THUMP; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR01213; pseudo_Pus10arc; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..407
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000407391"
FT   ACT_SITE        232
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         300
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         369
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
SQ   SEQUENCE   407 AA;  47445 MW;  FEC798DC947FA70D CRC64;
     MTEKEYNTRQ TQNYALCPKC LSRIYRNPKD RDNSIIPLIN NTQKCSICNN LLLNEDKIFK
     LILKKIKMLK IEFDTFLIAT QINNQTITKN QKEIYKITNY HGNNDIKHQI RRDISRLIEE
     KLGKTYDYKN PEVVIMVKIR KKPYKHNPYP EISNVNIFID SNPIFIEGKY RKLVRGIPQT
     KWPCTHCKGK GCEACDYTGQ QYKDTVEDLI SREILPMTNG NTTKFHGSGR EDIDVRMLGE
     GRPFVIEVKH PFKRKIDLKF LRVLVNSHSD GKIEINDLKY VTKERKASIK NSSVESYKIY
     SAIAEFENGV TSKDIYNIEK LKTIDQRTPI RVEHRRADLI RTREIKNIEV ERINSKKLHL
     IIKCQGGLYI KELISGDNNR TKPSVSSITN NQAECTQLDV LKVHIPE