ID   PUS10_THEAM             Reviewed;         441 AA.
AC   D5TZL3;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 1.
DT   03-AUG-2022, entry version 47.
DE   RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01893};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
DE            Short=Psi54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
GN   Name=pus10 {ECO:0000255|HAMAP-Rule:MF_01893}; OrderedLocusNames=Tagg_0031;
OS   Thermosphaera aggregans (strain DSM 11486 / M11TL).
OC   Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC   Desulfurococcaceae; Thermosphaera.
OX   NCBI_TaxID=633148;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 11486 / M11TL;
RX   DOI=10.4056/sigs.821804;
RA   Spring S., Rachel R., Lapidus A., Davenport K., Tice H., Copeland A.,
RA   Cheng J.-F., Lucas S., Chen F., Nolan M., Bruce D., Goodwin L., Pitluck S.,
RA   Ivanova N., Mavromatis K., Ovchinnikova G., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Chang Y.-J., Jeffries C.C., Brettin T.,
RA   Detter J.C., Tapia R., Han C., Heimerl T., Weikl F., Brambilla E.,
RA   Goker M., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.-P.;
RT   "Complete genome sequence of Thermosphaera aggregans type strain
RT   (M11TLT).";
RL   Stand. Genomic Sci. 2:245-259(2010).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01893}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01893};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01893};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01893}.
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DR   EMBL; CP001939; ADG90313.1; -; Genomic_DNA.
DR   RefSeq; WP_013128906.1; NC_014160.1.
DR   AlphaFoldDB; D5TZL3; -.
DR   SMR; D5TZL3; -.
DR   STRING; 633148.Tagg_0031; -.
DR   EnsemblBacteria; ADG90313; ADG90313; Tagg_0031.
DR   GeneID; 9165037; -.
DR   KEGG; tag:Tagg_0031; -.
DR   eggNOG; arCOG01015; Archaea.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   OMA; DGGLYIK; -.
DR   OrthoDB; 61936at2157; -.
DR   Proteomes; UP000002376; Chromosome.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR039894; Pus10-like.
DR   InterPro; IPR004114; THUMP_dom.
DR   PANTHER; PTHR21568; PTHR21568; 1.
DR   Pfam; PF02926; THUMP; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR01213; pseudo_Pus10arc; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..441
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000407399"
FT   ACT_SITE        268
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         333
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         405
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
SQ   SEQUENCE   441 AA;  50097 MW;  EB5D7A27719E34D7 CRC64;
     MGGSSISTPL DFNKIVEDVE KTLSKYPLCD HCLGRFFAKY GLGLSNRERG SSLKTMLGFK
     LYDDYSSKRI GRDSLLALAE NAGEPLTRMV EKLFAENVNP KTCFICGGRI SDEWLDSVAE
     AVYEKLKEAS VTRFVVGVKL GRNLREKELQ LVTETGFTRA ESLKNEIKRE VGKKVMAKYG
     LVPDFEKPEA TAIVKLDENF NYRVELVITP VLLKGVYNKR GRNISHVPWL AKQGGRKYPL
     SIQEYLESRL ADPFKAKKVK IHAAGREDVD ARTLGPGRPL VIEIVEPLVR SYDIEDVNKL
     IRSDLVEVRV HKPASRRDIE LLKQTSREKR KVYRLLVVSS TPISEAQLNE LEAYFNDIAI
     QQRTPIRILT RKKDVLRVRK VYEVRTKPVS STSFEALVYC DGGLYVKELV HCDQGRTNPC
     FASILNTELK PVELDVLYIE H