ID   PUS10_THEPD             Reviewed;         444 AA.
AC   A1RX33;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01893};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
DE            Short=Psi54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
GN   Name=pus10 {ECO:0000255|HAMAP-Rule:MF_01893}; OrderedLocusNames=Tpen_0354;
OS   Thermofilum pendens (strain DSM 2475 / Hrk 5).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermofilales; Thermofilaceae;
OC   Thermofilum.
OX   NCBI_TaxID=368408;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 2475 / Hrk 5;
RX   PubMed=18263724; DOI=10.1128/jb.01949-07;
RA   Anderson I., Rodriguez J., Susanti D., Porat I., Reich C., Ulrich L.E.,
RA   Elkins J.G., Mavromatis K., Lykidis A., Kim E., Thompson L.S., Nolan M.,
RA   Land M., Copeland A., Lapidus A., Lucas S., Detter C., Zhulin I.B.,
RA   Olsen G.J., Whitman W., Mukhopadhyay B., Bristow J., Kyrpides N.;
RT   "Genome sequence of Thermofilum pendens reveals an exceptional loss of
RT   biosynthetic pathways without genome reduction.";
RL   J. Bacteriol. 190:2957-2965(2008).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01893}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01893};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01893};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01893}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000505; ABL77763.1; -; Genomic_DNA.
DR   AlphaFoldDB; A1RX33; -.
DR   SMR; A1RX33; -.
DR   STRING; 368408.Tpen_0354; -.
DR   EnsemblBacteria; ABL77763; ABL77763; Tpen_0354.
DR   KEGG; tpe:Tpen_0354; -.
DR   eggNOG; arCOG01015; Archaea.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   OMA; DGGLYIK; -.
DR   Proteomes; UP000000641; Chromosome.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR039894; Pus10-like.
DR   InterPro; IPR004114; THUMP_dom.
DR   PANTHER; PTHR21568; PTHR21568; 2.
DR   Pfam; PF02926; THUMP; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR01213; pseudo_Pus10arc; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..444
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000407397"
FT   ACT_SITE        265
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         333
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         405
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
SQ   SEQUENCE   444 AA;  50205 MW;  9867C6736B3B0F2F CRC64;
     MSTGTSASYK RDNAFYALDK VERILLDGYS LCDACTGRLF GLRGYGLSNT ERGRALKTLL
     IMKAFQASPR QADLELLRVL ARTGFEPARE LLKKLSGEDV EVKACSICEG LTGRYYELAL
     RAVEEAKSYE FNTFEVGVRI DAEVIRREEE LWRRYGLESA ESIRNEASRE VGKIISKLTG
     KEYSRNNSEL LIIVDLSAGA IELHPAPVFV YGRYRKYARG LPQNPWPQPD ERIKFNTSIE
     ELIVKPALEL FEAEKAKFHA AGREDIDVRT LGTGRPFVLE IKKPRKRNID LKVLAEKINS
     GAGGLIEVLD LAYTDRKTIK KLKSLASIAK KAYVARVKFE KPVDDEKLAE ISKVFSNAVI
     NQRTPTRVLH RRVDKLRKKI VYRLEARKIS QDEVEFYLET QGGFYVKEFI HGDNGRTTPS
     IAEFLGNNVL SIELDVVSIE ETAA