ID   PUS10_VULDI             Reviewed;         412 AA.
AC   E1QUT8;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000255|HAMAP-Rule:MF_01893};
DE            EC=5.4.99.25 {ECO:0000255|HAMAP-Rule:MF_01893};
DE   AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
DE            Short=Psi54/55 synthase {ECO:0000255|HAMAP-Rule:MF_01893};
GN   Name=pus10 {ECO:0000255|HAMAP-Rule:MF_01893}; OrderedLocusNames=Vdis_0543;
OS   Vulcanisaeta distributa (strain DSM 14429 / JCM 11212 / NBRC 100878 /
OS   IC-017).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Vulcanisaeta.
OX   NCBI_TaxID=572478;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 14429 / JCM 11212 / NBRC 100878 / IC-017;
RX   DOI=10.4056/sigs.1113067;
RA   Mavromatis K., Sikorski J., Pabst E., Teshima H., Lapidus A., Lucas S.,
RA   Nolan M., Glavina Del Rio T., Cheng J., Bruce D., Goodwin L., Pitluck S.,
RA   Liolios K., Ivanova N., Mikhailova N., Pati A., Chen A., Palaniappan K.,
RA   Land M., Hauser L., Chang Y., Jeffries C., Rohde M., Spring S., Goker M.,
RA   Wirth R., Woyke T., Bristow J., Eisen J., Markowitz V., Hugenholtz P.,
RA   Klenk H., Kyrpides N.;
RT   "Complete genome sequence of Vulcanisaeta distributa type strain (IC-
RT   017T).";
RL   Stand. Genomic Sci. 3:117-125(2010).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC       uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000255|HAMAP-
CC       Rule:MF_01893}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC         Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01893};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC         Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01893};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01893}.
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DR   EMBL; CP002100; ADN49941.1; -; Genomic_DNA.
DR   RefSeq; WP_013335666.1; NC_014537.1.
DR   AlphaFoldDB; E1QUT8; -.
DR   SMR; E1QUT8; -.
DR   STRING; 572478.Vdis_0543; -.
DR   PRIDE; E1QUT8; -.
DR   EnsemblBacteria; ADN49941; ADN49941; Vdis_0543.
DR   GeneID; 9751463; -.
DR   KEGG; vdi:Vdis_0543; -.
DR   eggNOG; arCOG01015; Archaea.
DR   HOGENOM; CLU_028780_2_0_2; -.
DR   OMA; DGGLYIK; -.
DR   OrthoDB; 61936at2157; -.
DR   Proteomes; UP000006681; Chromosome.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01893; Pus10_arch; 1.
DR   InterPro; IPR005912; Pus10.
DR   InterPro; IPR039894; Pus10-like.
DR   InterPro; IPR004114; THUMP_dom.
DR   PANTHER; PTHR21568; PTHR21568; 1.
DR   Pfam; PF02926; THUMP; 1.
DR   TIGRFAMs; TIGR01213; pseudo_Pus10arc; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; tRNA processing.
FT   CHAIN           1..412
FT                   /note="tRNA pseudouridine synthase Pus10"
FT                   /id="PRO_0000407400"
FT   DOMAIN          73..197
FT                   /note="THUMP"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         308
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
FT   BINDING         376
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01893"
SQ   SEQUENCE   412 AA;  46904 MW;  8325E8CF5DD99316 CRC64;
     MSIERAMDIL RKYPLCNHCL GRLFARLGTG LDNEERGRAI KDYLLMRIHE RILNEGLSDE
     LLNDVKALAV SGHEPSIKFL SNMGINVSPA RCYVCGDTIF NRLNEWVNNI VNSLRSLGIE
     FRSFRLGSRV PLDIQNRELS ITTEFNISSA ESIKREINRE LGKRVSAMLG VSFNREEPDV
     EVVIDVNTGS VEVQIMPIYI SARYRKVHRL INEEGQVKWP IDRVIQAYNA QDVVIHTGGE
     DPMGVRVLGN GRPVILQVVK PSKRPDVNDV YALLKDSGYD IVLDNLSRVR ASAVVKMKAR
     VRDYVITYRV LAITDNSVTN ENIKSLHDYF RNRQVVQVFR RGRRIRRRIS MVYELDGRVI
     RDRLVEFLIR CQGNLYIRGF VHGGLGDVEP SIAGTLGFSV RPVEIDILNI SD