ID   PUS1_ARATH              Reviewed;         322 AA.
AC   Q7XA65; Q9C7K8; Q9C7Y0;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=RNA pseudouridine synthase 1;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase 1;
DE   AltName: Full=RNA-uridine isomerase 1;
GN   OrderedLocusNames=At1g56345; ORFNames=F13N6.19, F14G9.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG50905.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AAG51501.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AC058785; AAG51501.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AC069159; AAG50905.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE33381.1; -; Genomic_DNA.
DR   EMBL; BT010200; AAQ22669.1; -; mRNA.
DR   EMBL; AK228224; BAF00174.1; -; mRNA.
DR   PIR; D96605; D96605.
DR   RefSeq; NP_176031.2; NM_104514.3.
DR   AlphaFoldDB; Q7XA65; -.
DR   SMR; Q7XA65; -.
DR   IntAct; Q7XA65; 1.
DR   STRING; 3702.AT1G56345.1; -.
DR   PaxDb; Q7XA65; -.
DR   PRIDE; Q7XA65; -.
DR   ProteomicsDB; 225932; -.
DR   EnsemblPlants; AT1G56345.1; AT1G56345.1; AT1G56345.
DR   GeneID; 842088; -.
DR   Gramene; AT1G56345.1; AT1G56345.1; AT1G56345.
DR   KEGG; ath:AT1G56345; -.
DR   Araport; AT1G56345; -.
DR   TAIR; locus:2824464; AT1G56345.
DR   eggNOG; KOG1919; Eukaryota.
DR   HOGENOM; CLU_063147_0_0_1; -.
DR   InParanoid; Q7XA65; -.
DR   OMA; MIITKSH; -.
DR   OrthoDB; 1378690at2759; -.
DR   PhylomeDB; Q7XA65; -.
DR   BioCyc; ARA:AT1G56345-MON; -.
DR   PRO; PR:Q7XA65; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q7XA65; baseline and differential.
DR   Genevisible; Q7XA65; AT.
DR   GO; GO:0009982; F:pseudouridine synthase activity; TAS:TAIR.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..322
FT                   /note="RNA pseudouridine synthase 1"
FT                   /id="PRO_0000371422"
FT   ACT_SITE        120
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   322 AA;  35894 MW;  4AECC1932C8C6406 CRC64;
     MSLLRNFFSF TFSANSIITK PYFAIPIKAM EATSELNLNY PKPISAPPPP ISKDIELRRA
     MEASSKSSLF NLTRDDILYE DEYLMAVNKP KGVYCEAVLR SAPQIVLDSS SEYHLANRLD
     RDTSGVMIIT KSHKVAAKLV KAFTEHKIRK SYIALCIGSS PNWRRVTVSS GHGRSKHGAW
     RVYAALDVGR VLPGGSFVRD METTFEVVSV NSVKNESCEL EDVNHVIVAE GERELSCGGD
     DDDVVVVVRA FPRSGRTHQI RLHCQYLGIP IRGDVKYHGV YEWNGRTFEG HELHAECLSL
     DHPVTGDSIV IRAPLPYWAA GD