ID   PUS1_ORYSJ              Reviewed;         345 AA.
AC   Q2QNM3; A0A0P0YBE5;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=RNA pseudouridine synthase 1;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase 1;
DE   AltName: Full=RNA-uridine isomerase 1;
GN   OrderedLocusNames=Os12g0560500, LOC_Os12g37380;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG   The rice chromosomes 11 and 12 sequencing consortia;
RT   "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT   genes and recent gene duplications.";
RL   BMC Biol. 3:20-20(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; DP000011; ABA98930.1; -; Genomic_DNA.
DR   EMBL; AP008218; BAF30037.1; -; Genomic_DNA.
DR   EMBL; AP014968; BAT17638.1; -; Genomic_DNA.
DR   EMBL; AK061524; BAG87983.1; -; mRNA.
DR   RefSeq; XP_015618310.1; XM_015762824.1.
DR   AlphaFoldDB; Q2QNM3; -.
DR   SMR; Q2QNM3; -.
DR   STRING; 4530.OS12T0560500-01; -.
DR   PaxDb; Q2QNM3; -.
DR   EnsemblPlants; Os12t0560500-01; Os12t0560500-01; Os12g0560500.
DR   GeneID; 4352512; -.
DR   Gramene; Os12t0560500-01; Os12t0560500-01; Os12g0560500.
DR   KEGG; osa:4352512; -.
DR   eggNOG; KOG1919; Eukaryota.
DR   InParanoid; Q2QNM3; -.
DR   OMA; MIITKSH; -.
DR   OrthoDB; 1378690at2759; -.
DR   Proteomes; UP000000763; Chromosome 12.
DR   Proteomes; UP000059680; Chromosome 12.
DR   ExpressionAtlas; Q2QNM3; baseline and differential.
DR   Genevisible; Q2QNM3; OS.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; Reference proteome; RNA-binding.
FT   CHAIN           1..345
FT                   /note="RNA pseudouridine synthase 1"
FT                   /id="PRO_0000368022"
FT   ACT_SITE        134
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   345 AA;  37223 MW;  E75670321C0A41F4 CRC64;
     MTRLPLLLHS PRFAAALTTP PPPPLPPARR LVAAAAGGDL SLAMSAATGE YPVPVSPPYP
     AASKDVELRR AMTASARSAA YSSAPVVFED EWLAVVDKPA GVYCDALLSA LPCSAATLGD
     EATKPNLHLA NRLDRDTSGL MVITKCNKVA GKLVKAFTEH KVKKTYLALC IGYPPAWEKI
     KICSGHGRSK HGAWRVYAMS DVGRSLPGGS VVRDMSTRFE VLGINGKGQF REPSNFEVDE
     TESITVQEKA ADLTSDGDEK NSIILVRAYP QSGRTHQIRL HCQYLGFPIR GDVKYSGVIE
     WNGVDYDGHA LHAESLSFVH PVTGLPVTFR SPLPSWANEF ISTMA