PUS3_ORYSJ
ID PUS3_ORYSJ Reviewed; 475 AA.
AC Q0J4D4; A0A0P0XHV8; Q6YZW4; Q6YZW5;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=RNA pseudouridine synthase 3, mitochondrial;
DE EC=5.4.99.-;
DE AltName: Full=RNA pseudouridylate synthase 3;
DE AltName: Full=RNA-uridine isomerase 3;
DE Flags: Precursor;
GN OrderedLocusNames=Os08g0520100, LOC_Os08g40860;
GN ORFNames=OJ1003_A09.9/OJ1003_A09.10, P0689E12.33 /P0689E12.34;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD09699.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC Sequence=BAD09700.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC Sequence=BAD10434.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
CC Sequence=BAD10435.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to two different genes.; Evidence={ECO:0000305};
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DR EMBL; AP004622; BAD09699.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP004622; BAD09700.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005509; BAD10434.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP005509; BAD10435.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008214; BAF24179.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT06288.1; -; Genomic_DNA.
DR EMBL; AK120052; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015650456.1; XM_015794970.1.
DR AlphaFoldDB; Q0J4D4; -.
DR STRING; 4530.OS08T0520100-01; -.
DR PaxDb; Q0J4D4; -.
DR PRIDE; Q0J4D4; -.
DR EnsemblPlants; Os08t0520100-01; Os08t0520100-01; Os08g0520100.
DR GeneID; 4346062; -.
DR Gramene; Os08t0520100-01; Os08t0520100-01; Os08g0520100.
DR KEGG; osa:4346062; -.
DR eggNOG; KOG1919; Eukaryota.
DR InParanoid; Q0J4D4; -.
DR OMA; CSWIELC; -.
DR OrthoDB; 1378690at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR ExpressionAtlas; Q0J4D4; baseline and differential.
DR Genevisible; Q0J4D4; OS.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
PE 2: Evidence at transcript level;
KW Isomerase; Mitochondrion; Reference proteome; RNA-binding; Transit peptide.
FT TRANSIT 1..15
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 16..475
FT /note="RNA pseudouridine synthase 3, mitochondrial"
FT /id="PRO_0000368024"
FT DOMAIN 90..160
FT /note="S4 RNA-binding"
FT REGION 40..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 230
FT /evidence="ECO:0000250"
FT CONFLICT 64
FT /note="D -> V (in Ref. 4; AK120052)"
FT /evidence="ECO:0000305"
FT CONFLICT 122
FT /note="S -> N (in Ref. 4; AK120052)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 475 AA; 53324 MW; FF32A78A694ACB59 CRC64;
MLCRRRRVGA AVRWLSRLAP PAPAEADPVV VRVDGSNVAR LGKPKPGPRP RQLLSLPPFP
GGGDGDPLPG RKAAAPRRVT AVSWVKHYLA DVPQEVVQAH FNKRLVYSEC SDHEVSVETI
KSQKHHLKKI KHNDVMEPGM RIHLPVSVAE GEIKKRYETI PTATLHPNKD EIEYLRRLVI
HKDSAILVLN KPPKVPMKGN LPVHNSMDVL AAAALSYGNE EGPKLVHRLD RESSGLLLFG
RTKESFTRLH WLFTSVNLAK TNSQVWNAAC EAYMQRYWAL VIGTPKEREG IISAPLSKVL
LDDGKAERVI LAHPSGIDGA QEAVTAYRVM GPTIHGCSWI ELRPLTGRKH QLRVHCAEAL
GTPIVGDYKY GWFVHQRWKQ NPQPDFEPFT GEPYKLRRPE GLEIQKGSVL SKVPLLHLHC
REMVIPNIAK FLSSNGEWHE NGAPWSKEKP NLLRFIAPMP AHMKISWNIM SSYLV
