PUS3_YEAST
ID PUS3_YEAST Reviewed; 442 AA.
AC P31115; D6VTN0;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=tRNA pseudouridine(38/39) synthase;
DE EC=5.4.99.45;
DE AltName: Full=Depressed growth-rate protein DEG1;
DE AltName: Full=tRNA pseudouridine synthase 3;
DE AltName: Full=tRNA pseudouridylate synthase 3;
DE AltName: Full=tRNA-uridine isomerase 3;
GN Name=DEG1; Synonyms=HRM3, PUS3; OrderedLocusNames=YFL001W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2036682; DOI=10.1007/bf00362080;
RA Agostoni Carbone M.L., Solinas M., Sora S., Panzeri L.;
RT "A gene tightly linked to CEN6 is important for growth of Saccharomyces
RT cerevisiae.";
RL Curr. Genet. 19:1-8(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=8789262;
RX DOI=10.1002/(sici)1097-0061(199601)12:1<77::aid-yea887>3.0.co;2-5;
RA Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T.,
RA Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T.,
RA Murakami Y.;
RT "Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome
RT VI.";
RL Yeast 12:77-84(1996).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBSTRATE SPECIFICITY.
RX PubMed=9430663; DOI=10.1074/jbc.273.3.1316;
RA Lecointe F., Simos G., Sauer A., Hurt E.C., Motorin Y., Grosjean H.;
RT "Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3)
RT catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop.";
RL J. Biol. Chem. 273:1316-1323(1998).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Formation of pseudouridines at positions 38 and 39 in the
CC anticodon stem and loop of transfer RNAs. {ECO:0000269|PubMed:9430663}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(38/39) in tRNA = pseudouridine(38/39) in tRNA;
CC Xref=Rhea:RHEA:42564, Rhea:RHEA-COMP:10117, Rhea:RHEA-COMP:10118,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.45;
CC Evidence={ECO:0000269|PubMed:9430663};
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 5860 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000305}.
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DR EMBL; D50617; BAA09239.1; -; Genomic_DNA.
DR EMBL; AY693197; AAT93216.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12440.1; -; Genomic_DNA.
DR PIR; S14145; S14145.
DR RefSeq; NP_116655.1; NM_001179965.1.
DR AlphaFoldDB; P31115; -.
DR SMR; P31115; -.
DR BioGRID; 31148; 420.
DR DIP; DIP-5151N; -.
DR IntAct; P31115; 7.
DR STRING; 4932.YFL001W; -.
DR iPTMnet; P31115; -.
DR MaxQB; P31115; -.
DR PaxDb; P31115; -.
DR PRIDE; P31115; -.
DR EnsemblFungi; YFL001W_mRNA; YFL001W; YFL001W.
DR GeneID; 850550; -.
DR KEGG; sce:YFL001W; -.
DR SGD; S000001895; DEG1.
DR VEuPathDB; FungiDB:YFL001W; -.
DR eggNOG; KOG2554; Eukaryota.
DR GeneTree; ENSGT00950000183160; -.
DR HOGENOM; CLU_014673_2_0_1; -.
DR InParanoid; P31115; -.
DR OMA; NLFRCDF; -.
DR BioCyc; MetaCyc:G3O-30453-MON; -.
DR BioCyc; YEAST:G3O-30453-MON; -.
DR BRENDA; 5.4.99.12; 984.
DR BRENDA; 5.4.99.45; 984.
DR PRO; PR:P31115; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P31115; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0009982; F:pseudouridine synthase activity; IDA:SGD.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IMP:SGD.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IDA:SGD.
DR CDD; cd02569; PseudoU_synth_ScPus3; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041707; Pus3-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00071; hisT_truA; 1.
PE 1: Evidence at protein level;
KW Isomerase; Nucleus; Reference proteome; tRNA processing.
FT CHAIN 1..442
FT /note="tRNA pseudouridine(38/39) synthase"
FT /id="PRO_0000057519"
FT ACT_SITE 151
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 222
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 442 AA; 50889 MW; 89C35917366BDB9B CRC64;
MSNFIRRLVG KMKAISTGTN AIVSKKDSIY ANWSKEQLIR RITELENANK PHSEKFQHIE
DNKKRKISQE EVTRSKAKKA PKKFDFSKHN TRFIALRFAY LGWNYNGLAV QKEYTPLPTV
EGTILEAMNK CKLVPSMVLQ DYKFSRCGRT DKGVSAMNQV ISLEVRSNLT DEEQRDPTND
SREIPYVHVL NQLLPDDIRI SAVCLRPPPN FDARFSCVHR HYKYIFNGKN LNIEKMSKAA
SYFVGERDFR NFCKLDGSKQ ITNFKRTIIS SKILPLSETF YCFDLVGSAF LWHQVRCMMA
ILFLVGQSLE VPEIVLRLTD IEKTPQRPVY EMANDIPLLL YDCKFPEMDW QEPTVDDYKA
IKFTTATEAL TLHYELKAAV CNIFKDVLPT ANTNNFSKTI INLGDGRGKV VGTYVKLEDR
SVMEPVEVVN AKYSKKKNNK NK
