ID   PUS4_ARATH              Reviewed;         477 AA.
AC   Q9LT72;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=RNA pseudouridine synthase 4, mitochondrial;
DE            EC=5.4.99.-;
DE   AltName: Full=RNA pseudouridylate synthase 4;
DE   AltName: Full=RNA-uridine isomerase 4;
DE   Flags: Precursor;
GN   OrderedLocusNames=At3g19440; ORFNames=MLD14.18;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Kim C.J., Bautista V.R., Chen H., De Los Reyes C., Wu S.Y., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AB025624; BAB02469.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE76240.1; -; Genomic_DNA.
DR   EMBL; AK228911; BAF00800.1; -; mRNA.
DR   EMBL; BT030392; ABO45695.1; -; mRNA.
DR   RefSeq; NP_188575.1; NM_112831.5.
DR   AlphaFoldDB; Q9LT72; -.
DR   SMR; Q9LT72; -.
DR   STRING; 3702.AT3G19440.1; -.
DR   iPTMnet; Q9LT72; -.
DR   PaxDb; Q9LT72; -.
DR   PRIDE; Q9LT72; -.
DR   ProteomicsDB; 224794; -.
DR   EnsemblPlants; AT3G19440.1; AT3G19440.1; AT3G19440.
DR   GeneID; 821478; -.
DR   Gramene; AT3G19440.1; AT3G19440.1; AT3G19440.
DR   KEGG; ath:AT3G19440; -.
DR   Araport; AT3G19440; -.
DR   TAIR; locus:2090689; AT3G19440.
DR   eggNOG; KOG1919; Eukaryota.
DR   HOGENOM; CLU_016902_13_0_1; -.
DR   InParanoid; Q9LT72; -.
DR   OrthoDB; 1378690at2759; -.
DR   PhylomeDB; Q9LT72; -.
DR   BioCyc; ARA:AT3G19440-MON; -.
DR   PRO; PR:Q9LT72; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LT72; baseline and differential.
DR   Genevisible; Q9LT72; AT.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; Mitochondrion; Reference proteome; RNA-binding; Transit peptide.
FT   TRANSIT         1..43
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           44..477
FT                   /note="RNA pseudouridine synthase 4, mitochondrial"
FT                   /id="PRO_0000371424"
FT   DOMAIN          90..175
FT                   /note="S4 RNA-binding"
FT   REGION          34..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        34..53
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        236
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   477 AA;  52965 MW;  104CE69B8059AA43 CRC64;
     MAKWRLATAT LRRQLQSSSP TISTFKNPTK ALSAAAHQST RSYSTTQTDD SRGKWLTLPP
     FSPTIDGTAV GKDLLSDGDS VKSSTDNSKT TALRWILRCR PDLPRTLVQK LFRLRQVRRE
     MSMSVDGDEL QRSQLKRVAA KESLNVGDRI YLPLSVDNDT PQTPPAKKES FQCSDEERKF
     VCSLVLYKDP AIIVLNKPHG LAVQGGSGIK TSIDELAASC LKFDKSESPR LVHRLDRDCS
     GLLVLARTQT AATVLHSIFR EKTTGASAYG VKKNVKSLKR KYMALVIGCP PRQRGQISAP
     LRKVVVDDGK SERITVNDNG ELVSTQHAIT EYRVIESSPH GYTWLELRPL TGRKHQLRVH
     CAEVLGTPIV GDYKYGWQAH KAREPFVSSE NNPTKQSSSP FGLDLDGGDV SSKQPHLHLH
     SKQIDLPNIS QLLEKMQVSS DSDISDLDSL KFDAPLPSHM QLSFNLLKSR VETCDKN