ID   PUS5_ASHGO              Reviewed;         260 AA.
AC   Q750S3;
DT   08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=21S rRNA pseudouridine(2819) synthase;
DE            EC=5.4.99.43 {ECO:0000250|UniProtKB:Q06244};
DE   AltName: Full=Pseudouridine synthase 5;
DE   AltName: Full=Pseudouridylate synthase PUS5;
DE   AltName: Full=Uracil hydrolyase PUS5;
GN   Name=PUS5; OrderedLocusNames=AGL134C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Pseudouridylate synthase responsible for the pseudouridine-
CC       2819 formation in mitochondrial 21S rRNA. May modulate the efficiency
CC       or the fidelity of the mitochondrial translation machinery.
CC       {ECO:0000250|UniProtKB:Q06244}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2819) in 21S rRNA = pseudouridine(2819) in 21S rRNA;
CC         Xref=Rhea:RHEA:42556, Rhea:RHEA-COMP:10113, Rhea:RHEA-COMP:10114,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.43;
CC         Evidence={ECO:0000250|UniProtKB:Q06244};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q06244}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; AE016820; AAS54357.1; -; Genomic_DNA.
DR   RefSeq; NP_986533.1; NM_211595.1.
DR   AlphaFoldDB; Q750S3; -.
DR   SMR; Q750S3; -.
DR   STRING; 33169.AAS54357; -.
DR   EnsemblFungi; AAS54357; AAS54357; AGOS_AGL134C.
DR   GeneID; 4622832; -.
DR   KEGG; ago:AGOS_AGL134C; -.
DR   eggNOG; KOG1919; Eukaryota.
DR   HOGENOM; CLU_081037_0_0_1; -.
DR   InParanoid; Q750S3; -.
DR   OMA; ACIITKI; -.
DR   Proteomes; UP000000591; Chromosome VII.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0004730; F:pseudouridylate synthase activity; IEA:EnsemblFungi.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Mitochondrion; Reference proteome; rRNA processing.
FT   CHAIN           1..260
FT                   /note="21S rRNA pseudouridine(2819) synthase"
FT                   /id="PRO_0000162749"
FT   ACT_SITE        68
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ   SEQUENCE   260 AA;  29476 MW;  EFF0765D04A8F447 CRC64;
     MNRWAVPILH EHKHYYIVNK VHGIVCQPPD LRTWYKYHDY EPPVLLDLLR KQHPNFGGEV
     WRTVHRLDEP VTGGVLVSRN KRAAAMFSRS LALGGNRGFP LTRRYVALLA REAKGLPSEG
     RITMGDMITD YKRLENDLVL LQLQTGRKHQ IRKQMAQVFG QPVVNDKMYG GDSVDGIVDN
     LIGLHSAFIG AQCGLQARTY LIPIPRTQDA FKLWDKYIDE QGGFIPSVQK ELRDFSLPSK
     LENTITLLSG GQGGIQISYK