ID   PUS5_CANAL              Reviewed;         248 AA.
AC   Q5A0Y2; A0A1D8PMQ9;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 2.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=21S rRNA pseudouridine(2819) synthase;
DE            EC=5.4.99.43 {ECO:0000250|UniProtKB:Q06244};
DE   AltName: Full=Pseudouridine synthase 5;
DE   AltName: Full=Pseudouridylate synthase PUS5;
DE   AltName: Full=Uracil hydrolyase PUS5;
GN   Name=PUS5; OrderedLocusNames=CAALFM_C406950WA;
GN   ORFNames=CaO19.10626, CaO19.3114;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- FUNCTION: Pseudouridylate synthase responsible for the pseudouridine-
CC       2819 formation in mitochondrial 21S rRNA. May modulate the efficiency
CC       or the fidelity of the mitochondrial translation machinery.
CC       {ECO:0000250|UniProtKB:Q06244}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2819) in 21S rRNA = pseudouridine(2819) in 21S rRNA;
CC         Xref=Rhea:RHEA:42556, Rhea:RHEA-COMP:10113, Rhea:RHEA-COMP:10114,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.43;
CC         Evidence={ECO:0000250|UniProtKB:Q06244};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q06244}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; CP017626; AOW29416.1; -; Genomic_DNA.
DR   RefSeq; XP_715423.2; XM_710330.2.
DR   AlphaFoldDB; Q5A0Y2; -.
DR   SMR; Q5A0Y2; -.
DR   STRING; 237561.Q5A0Y2; -.
DR   GeneID; 3642927; -.
DR   KEGG; cal:CAALFM_C406950WA; -.
DR   CGD; CAL0000185188; orf19.10626.
DR   VEuPathDB; FungiDB:C4_06950W_A; -.
DR   eggNOG; KOG1919; Eukaryota.
DR   HOGENOM; CLU_998077_0_0_1; -.
DR   InParanoid; Q5A0Y2; -.
DR   OrthoDB; 1378690at2759; -.
DR   PRO; PR:Q5A0Y2; -.
DR   Proteomes; UP000000559; Chromosome 4.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Isomerase; Mitochondrion; Reference proteome; rRNA processing.
FT   CHAIN           1..248
FT                   /note="21S rRNA pseudouridine(2819) synthase"
FT                   /id="PRO_0000162750"
FT   ACT_SITE        58
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ   SEQUENCE   248 AA;  28391 MW;  2612EC87517DF232 CRC64;
     MKLDLVKRTF NYAVVNKPSG MVCDASHTNN IITALTNEFT KILPSVNSSQ FRLVQRLDRF
     VTGGLVVARN KKWADKVRKS FFQEGTLRLT RRYVGLIALD QIPESTQGTI DFPIQALEKD
     YRGKNKSREL FTYSAVTHYK LIPSARRTIK GVFPVFQQGP ILPIILELET GRKNQIRDHI
     IQKFGVPLLN DDNFSDFKLN SEIPKDVNSK LYKSNQIALH SGLVIMENNG ISQQFLFPVN
     NAYDRELW