ID   PUS5_CANGA              Reviewed;         223 AA.
AC   Q6FS81;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=21S rRNA pseudouridine(2819) synthase;
DE            EC=5.4.99.43 {ECO:0000250|UniProtKB:Q06244};
DE   AltName: Full=Pseudouridine synthase 5;
DE   AltName: Full=Pseudouridylate synthase PUS5;
DE   AltName: Full=Uracil hydrolyase PUS5;
GN   Name=PUS5; OrderedLocusNames=CAGL0H02717g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Pseudouridylate synthase responsible for the pseudouridine-
CC       2819 formation in mitochondrial 21S rRNA. May modulate the efficiency
CC       or the fidelity of the mitochondrial translation machinery.
CC       {ECO:0000250|UniProtKB:Q06244}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2819) in 21S rRNA = pseudouridine(2819) in 21S rRNA;
CC         Xref=Rhea:RHEA:42556, Rhea:RHEA-COMP:10113, Rhea:RHEA-COMP:10114,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.43;
CC         Evidence={ECO:0000250|UniProtKB:Q06244};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q06244}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CR380954; CAG59846.1; -; Genomic_DNA.
DR   RefSeq; XP_446913.1; XM_446913.1.
DR   AlphaFoldDB; Q6FS81; -.
DR   SMR; Q6FS81; -.
DR   STRING; 5478.XP_446913.1; -.
DR   EnsemblFungi; CAG59846; CAG59846; CAGL0H02717g.
DR   GeneID; 2888440; -.
DR   KEGG; cgr:CAGL0H02717g; -.
DR   CGD; CAL0131838; CAGL0H02717g.
DR   VEuPathDB; FungiDB:CAGL0H02717g; -.
DR   eggNOG; KOG1919; Eukaryota.
DR   HOGENOM; CLU_081037_0_0_1; -.
DR   InParanoid; Q6FS81; -.
DR   OMA; ACIITKI; -.
DR   Proteomes; UP000002428; Chromosome H.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004730; F:pseudouridylate synthase activity; IEA:EnsemblFungi.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:EnsemblFungi.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   3: Inferred from homology;
KW   Isomerase; Mitochondrion; Reference proteome; rRNA processing.
FT   CHAIN           1..223
FT                   /note="21S rRNA pseudouridine(2819) synthase"
FT                   /id="PRO_0000162751"
FT   ACT_SITE        58
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ   SEQUENCE   223 AA;  24814 MW;  740A797F6E019F10 CRC64;
     MSARLKVVYD GMHYMIVYKS AGVLSQPGLA SDSRPVLMSE LRGLLSETLD LYSVQRLDAC
     VTGGTVVAKN KRAAMMFSRY LKQGGGSGYG LTRRYLARIN AQPVCDEGTI ESPGMVTYYR
     RVRDDCVVVE LKTGKKHQIR KHLALNLNCP IVNDTYYGGS VVKGVNGQIA LHSAFVRTRI
     GKNMQDHLVG IEPQEQTLWK SILTENGMLP DDIVRTLTRD KLF