ID   PUS5_KLULA              Reviewed;         299 AA.
AC   Q6CQC4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=21S rRNA pseudouridine(2819) synthase;
DE            EC=5.4.99.43 {ECO:0000250|UniProtKB:Q06244};
DE   AltName: Full=Pseudouridine synthase 5;
DE   AltName: Full=Pseudouridylate synthase PUS5;
DE   AltName: Full=Uracil hydrolyase PUS5;
GN   Name=PUS5; OrderedLocusNames=KLLA0D18172g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Pseudouridylate synthase responsible for the pseudouridine-
CC       2819 formation in mitochondrial 21S rRNA. May modulate the efficiency
CC       or the fidelity of the mitochondrial translation machinery.
CC       {ECO:0000250|UniProtKB:Q06244}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(2819) in 21S rRNA = pseudouridine(2819) in 21S rRNA;
CC         Xref=Rhea:RHEA:42556, Rhea:RHEA-COMP:10113, Rhea:RHEA-COMP:10114,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.43;
CC         Evidence={ECO:0000250|UniProtKB:Q06244};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q06244}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; CR382124; CAH00961.1; -; Genomic_DNA.
DR   RefSeq; XP_453865.1; XM_453865.1.
DR   AlphaFoldDB; Q6CQC4; -.
DR   SMR; Q6CQC4; -.
DR   STRING; 28985.XP_453865.1; -.
DR   EnsemblFungi; CAH00961; CAH00961; KLLA0_D18172g.
DR   GeneID; 2893481; -.
DR   KEGG; kla:KLLA0_D18172g; -.
DR   eggNOG; KOG1919; Eukaryota.
DR   HOGENOM; CLU_081037_0_0_1; -.
DR   InParanoid; Q6CQC4; -.
DR   OMA; ACIITKI; -.
DR   Proteomes; UP000000598; Chromosome D.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   3: Inferred from homology;
KW   Isomerase; Mitochondrion; Reference proteome; rRNA processing.
FT   CHAIN           1..299
FT                   /note="21S rRNA pseudouridine(2819) synthase"
FT                   /id="PRO_0000162753"
FT   ACT_SITE        106
FT                   /evidence="ECO:0000250|UniProtKB:P0AA37"
SQ   SEQUENCE   299 AA;  34308 MW;  CCAF647D2AF82C1B CRC64;
     MLRRGFIKQP PFVKIFKIVS PESTNAKATH CEVEFPLKVQ ELWRDKHHVI FNKPPLALSQ
     LPDKRTWYNT HDYDPPVLLD IVQSSSGSIL KDGTYEPSYY PIHRIDTPVS GGIVYAVNKQ
     SAQQFSRNLR YGGNKGFSLT RKYVAKVGKS KTANECKEGL IKWNGAITYF QRVDNEHLIL
     QLVTGKKHQI RKLTQQVLDS PIYNDLKYGG KKVFDSDFQI ALHSAYIRTK IGFNIHEQII
     PVPDGFRMIW GESVDQNGNF NEDITRVLKE DWAGTIKECL KKLKIQETKL ADNQLIFVS