ID   PUS7L_DANRE             Reviewed;         643 AA.
AC   Q1L8I0; A4QN40;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Pseudouridylate synthase PUS7L {ECO:0000305};
DE            EC=5.4.99.- {ECO:0000250|UniProtKB:Q9H0K6};
DE   AltName: Full=Pseudouridylate synthase 7 homolog-like protein {ECO:0000305};
GN   Name=pus7l; ORFNames=si:dkey-234l24.3;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=WIK; TISSUE=Ovary;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Pseudouridine synthase that catalyzes pseudouridylation of
CC       mRNAs. {ECO:0000250|UniProtKB:Q9H0K6}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in mRNA = a pseudouridine in mRNA;
CC         Xref=Rhea:RHEA:56644, Rhea:RHEA-COMP:14658, Rhea:RHEA-COMP:14659,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC         Evidence={ECO:0000250|UniProtKB:Q9H0K6};
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC       {ECO:0000305}.
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DR   EMBL; CR786571; CAK10756.1; -; Genomic_DNA.
DR   EMBL; BC134851; AAI34852.1; -; mRNA.
DR   RefSeq; NP_001038422.1; NM_001044957.2.
DR   AlphaFoldDB; Q1L8I0; -.
DR   SMR; Q1L8I0; -.
DR   STRING; 7955.ENSDARP00000092588; -.
DR   PaxDb; Q1L8I0; -.
DR   Ensembl; ENSDART00000143804; ENSDARP00000115135; ENSDARG00000069780.
DR   GeneID; 561376; -.
DR   KEGG; dre:561376; -.
DR   CTD; 83448; -.
DR   ZFIN; ZDB-GENE-050419-188; pus7l.
DR   eggNOG; KOG2339; Eukaryota.
DR   GeneTree; ENSGT00530000063554; -.
DR   InParanoid; Q1L8I0; -.
DR   OrthoDB; 955294at2759; -.
DR   PhylomeDB; Q1L8I0; -.
DR   PRO; PR:Q1L8I0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 4.
DR   Bgee; ENSDARG00000069780; Expressed in tail and 20 other tissues.
DR   ExpressionAtlas; Q1L8I0; baseline.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0009982; F:pseudouridine synthase activity; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; ISS:UniProtKB.
DR   GO; GO:0001522; P:pseudouridine synthesis; IBA:GO_Central.
DR   Gene3D; 3.30.2350.20; -; 2.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001656; PsdUridine_synth_TruD.
DR   InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR   InterPro; IPR042214; TruD_catalytic.
DR   PANTHER; PTHR13326; PTHR13326; 2.
DR   Pfam; PF01142; TruD; 1.
DR   PIRSF; PIRSF037016; Pseudouridin_synth_euk_prd; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   TIGRFAMs; TIGR00094; tRNA_TruD_broad; 1.
DR   PROSITE; PS50984; TRUD; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; mRNA processing; Reference proteome.
FT   CHAIN           1..643
FT                   /note="Pseudouridylate synthase PUS7L"
FT                   /id="PRO_0000316787"
FT   DOMAIN          370..597
FT                   /note="TRUD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00342"
FT   ACT_SITE        284
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q57261"
FT   CONFLICT        169
FT                   /note="F -> L (in Ref. 2; AAI34852)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        243
FT                   /note="Missing (in Ref. 2; AAI34852)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        316
FT                   /note="E -> Q (in Ref. 2; AAI34852)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        435
FT                   /note="V -> A (in Ref. 2; AAI34852)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        451
FT                   /note="L -> I (in Ref. 2; AAI34852)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        530
FT                   /note="T -> A (in Ref. 2; AAI34852)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        559
FT                   /note="L -> I (in Ref. 2; AAI34852)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        607
FT                   /note="T -> R (in Ref. 2; AAI34852)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   643 AA;  73112 MW;  C5946EF486C15085 CRC64;
     MERNTLSCFI SDHEGFNGTI KNSPQDFVVI EIDTNGQFVN SVNTEEEERP CQTTKRIGKL
     KPSKPDDTDS LIQDCFDLNL ILGLSVNREL EHFTNKLKVK PCSDDQEKVE LSLGTFPDKH
     MRAVVHKAVR FNFPFLQTLT NQSEIRVRED PDFQELAGLA SEGEAEDFFR FIDAKTPGSV
     FTFLPDDSKE HRTSVHHFVS RRFGKLVETK SFVDQQKTCI TVRLRERGKQ AKKRTMADCQ
     MQEESLYTAF TLRKENLETL EAISYMAAVL GVLPSDFTYA GIKDKRAITY QAMVVKKISP
     ERLLEKGSEF ERRGMEISRV RPAFEALKLG RLQGNHFELV IRDLKHHGKH GLAELPKVIE
     EAVENVKNKG FVNYYGPQRF GSGSCVQADQ VGLALLKENM EASVKLFFTP EDDDDLQNKA
     KRHFLHTGNA KESLVLMPAY KARERLMLRA LHRYGSGQEG CTRGWLSLPH SMRVFYLHSY
     CSRVWNQAAK YRLQKLGFKP VQGDLVWAEP VKGLKDATEE LSAPQIHVVT SDEEKNEVFS
     LDQVILPMPG NSVKYPENLL GQWYQDRLAQ DGLESCRFRV TPLKLNVPGC YRPLLAKPQN
     ITYSLQTSSS DESNTHCLSL NFDLEASCYA TVCLGEIMKT NLS