PUS7L_MOUSE
ID PUS7L_MOUSE Reviewed; 702 AA.
AC Q8CE46; Q6NY03; Q8C9X5;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Pseudouridylate synthase PUS7L {ECO:0000305};
DE EC=5.4.99.- {ECO:0000250|UniProtKB:Q9H0K6};
DE AltName: Full=Pseudouridylate synthase 7 homolog-like protein {ECO:0000305};
GN Name=Pus7l {ECO:0000312|MGI:MGI:1926145};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Skin, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Pseudouridine synthase that catalyzes pseudouridylation of
CC mRNAs. {ECO:0000250|UniProtKB:Q9H0K6}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in mRNA = a pseudouridine in mRNA;
CC Xref=Rhea:RHEA:56644, Rhea:RHEA-COMP:14658, Rhea:RHEA-COMP:14659,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000250|UniProtKB:Q9H0K6};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruD family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC30554.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact. May result from internal priming due to genomic poly-A tracts.; Evidence={ECO:0000305};
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DR EMBL; AK029027; BAC26251.1; -; mRNA.
DR EMBL; AK040257; BAC30554.1; ALT_SEQ; mRNA.
DR EMBL; BC066798; AAH66798.1; -; mRNA.
DR CCDS; CCDS27771.1; -.
DR RefSeq; NP_766025.1; NM_172437.3.
DR AlphaFoldDB; Q8CE46; -.
DR SMR; Q8CE46; -.
DR BioGRID; 219690; 2.
DR STRING; 10090.ENSMUSP00000044075; -.
DR iPTMnet; Q8CE46; -.
DR PhosphoSitePlus; Q8CE46; -.
DR EPD; Q8CE46; -.
DR MaxQB; Q8CE46; -.
DR PaxDb; Q8CE46; -.
DR PeptideAtlas; Q8CE46; -.
DR PRIDE; Q8CE46; -.
DR ProteomicsDB; 301899; -.
DR Antibodypedia; 25131; 49 antibodies from 14 providers.
DR DNASU; 78895; -.
DR Ensembl; ENSMUST00000049151; ENSMUSP00000044075; ENSMUSG00000033356.
DR GeneID; 78895; -.
DR KEGG; mmu:78895; -.
DR UCSC; uc007xjh.2; mouse.
DR CTD; 83448; -.
DR MGI; MGI:1926145; Pus7l.
DR VEuPathDB; HostDB:ENSMUSG00000033356; -.
DR eggNOG; KOG2339; Eukaryota.
DR GeneTree; ENSGT00530000063554; -.
DR HOGENOM; CLU_005281_1_1_1; -.
DR InParanoid; Q8CE46; -.
DR OMA; FEPDGNK; -.
DR OrthoDB; 955294at2759; -.
DR PhylomeDB; Q8CE46; -.
DR TreeFam; TF314278; -.
DR BioGRID-ORCS; 78895; 2 hits in 74 CRISPR screens.
DR PRO; PR:Q8CE46; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8CE46; protein.
DR Bgee; ENSMUSG00000033356; Expressed in ear vesicle and 165 other tissues.
DR ExpressionAtlas; Q8CE46; baseline and differential.
DR Genevisible; Q8CE46; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009982; F:pseudouridine synthase activity; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; ISS:UniProtKB.
DR GO; GO:0001522; P:pseudouridine synthesis; IBA:GO_Central.
DR Gene3D; 3.30.2350.20; -; 2.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001656; PsdUridine_synth_TruD.
DR InterPro; IPR011760; PsdUridine_synth_TruD_insert.
DR InterPro; IPR042214; TruD_catalytic.
DR PANTHER; PTHR13326; PTHR13326; 1.
DR Pfam; PF01142; TruD; 1.
DR PIRSF; PIRSF037016; Pseudouridin_synth_euk_prd; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00094; tRNA_TruD_broad; 1.
DR PROSITE; PS50984; TRUD; 1.
PE 1: Evidence at protein level;
KW Isomerase; mRNA processing; Phosphoprotein; Reference proteome.
FT CHAIN 1..702
FT /note="Pseudouridylate synthase PUS7L"
FT /id="PRO_0000316786"
FT DOMAIN 424..646
FT /note="TRUD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00342"
FT REGION 84..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 339
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q57261"
FT MOD_RES 79
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9H0K6"
FT CONFLICT 151
FT /note="S -> P (in Ref. 2; AAH66798)"
FT /evidence="ECO:0000305"
FT CONFLICT 558
FT /note="D -> N (in Ref. 2; AAH66798)"
FT /evidence="ECO:0000305"
FT CONFLICT 667
FT /note="E -> G (in Ref. 2; AAH66798)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 702 AA; 79197 MW; 77D12455B78BCAEE CRC64;
MAEDTDGGIR FNSLCFINDH VGFQGSIKTS PSDFIVIEID EQGQLVSKAT DGSLYEISKI
QSEPSNSVKK PKLNIQNVSL EHKNSEGAAD LPGCSDGDRS HQSDSEKENS VNSVTSKCEE
ESVDLLRSLL DEKTHTSLGQ FACDIKRMWN SQTELTEPSP ELSLGKILDK NRRAVLHSAV
RQAFPFLITV GNQGEVVVKP NRECKELCRL VSEEEALGFF KYLDAKKENS KFTFKPDPNK
DHRKAVHHFL NKKFGNLVET KSFPGQHHSA GNPDSAITVR FREKARGKRS HPEGCERGKA
VYTAFTLQKE NLETFEAIGL LAVKLGVIPS DFSYAGLKDK RAITYQSMVV KKVTPERLKS
IKEEIEKKRM NVFNIRSVGD CLRLGQLKGN HFEIIIRHLR NQLNDSANLT ERILEAIENV
KNKGFVNYYG PQRFGKGQKI QTDQIGLALL KNEMVKAIKL FLTPEDVDDP VNKAKQYFLR
TEDAKGTLSL MPEFRVRERA LLESLHRFGV TEEGCVRAWF SFPHSMRIFY IHAYSSRIWN
EAASYRLAAY GPKVVEGDLI CSDEDADKHF PSSKVHLVTK EEESAHTYAL HQVVLPVLGY
NVQYPENQVG RWYQEALSRD GLQACRFRVP ALKLNVPGCY RHIVKHPRNV SHRLVHPDPA
TEEARVEGPH SDDTASSLSL SFDLDASCYA TVCLREMMKG DI
