PUS7_ARATH
ID PUS7_ARATH Reviewed; 395 AA.
AC Q9LU60; Q8LG24; Q8W4Q8;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=RNA pseudouridine synthase 7;
DE EC=5.4.99.-;
DE AltName: Full=RNA pseudouridylate synthase 7;
DE AltName: Full=RNA-uridine isomerase 7;
GN OrderedLocusNames=At5g51140; ORFNames=MWD22.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 13-395.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9LU60-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL31256.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB023044; BAA97376.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96042.1; -; Genomic_DNA.
DR EMBL; AY084499; AAM61068.1; -; mRNA.
DR EMBL; AY061753; AAL31256.1; ALT_INIT; mRNA.
DR EMBL; BT000855; AAN38692.1; -; mRNA.
DR RefSeq; NP_199927.1; NM_124493.4. [Q9LU60-1]
DR AlphaFoldDB; Q9LU60; -.
DR SMR; Q9LU60; -.
DR STRING; 3702.AT5G51140.2; -.
DR iPTMnet; Q9LU60; -.
DR PaxDb; Q9LU60; -.
DR PRIDE; Q9LU60; -.
DR EnsemblPlants; AT5G51140.1; AT5G51140.1; AT5G51140. [Q9LU60-1]
DR GeneID; 835188; -.
DR Gramene; AT5G51140.1; AT5G51140.1; AT5G51140. [Q9LU60-1]
DR KEGG; ath:AT5G51140; -.
DR Araport; AT5G51140; -.
DR eggNOG; KOG1919; Eukaryota.
DR HOGENOM; CLU_016902_12_4_1; -.
DR InParanoid; Q9LU60; -.
DR OMA; CYRLDKI; -.
DR PhylomeDB; Q9LU60; -.
DR PRO; PR:Q9LU60; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LU60; baseline and differential.
DR Genevisible; Q9LU60; AT.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IBA:GO_Central.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Isomerase; Reference proteome; RNA-binding.
FT CHAIN 1..395
FT /note="RNA pseudouridine synthase 7"
FT /id="PRO_0000371427"
FT DOMAIN 74..136
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 244..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 187
FT /evidence="ECO:0000250"
FT CONFLICT 182
FT /note="P -> S (in Ref. 3; AAM61068)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 395 AA; 44625 MW; 005C362A695A7670 CRC64;
MKRKQQEDDN DDGVEKAVSP VLATTSNTMS TDLLLQSSSK LSQKQDYIFH GGRRHVRPYY
FEFISHVNKR WTGKTIVDLF ADEFKGRPRD YYVGAVKSGR IKVDGEIVPV SYIVKSSQKI
THFLHRHEPP VMIDDVVILH QEPDVVTVCK PASVPVHPCG QYRKNTIVGI LDAEHDLGTL
FPIHRLDRLV SGLLIIARTA AKADFFRQQI EGGMVKKRYI AKVIGVFPED EMIVDANINY
NGSEGRSTAE DANSSGDDKK VKGKPACTKF TRIDTNGTHS LVSCEPVTGR THQIRVHLQY
TGHPIANDPL YLNQHIDNLE TYIAKRIDAG ERKIVSPDDY VYSSEDFSID PMCTNCPKLI
PQGYEEHDEA LWLHCVQYCG TGWEYECPYP SWASL
