PUS9_YEAST
ID PUS9_YEAST Reviewed; 462 AA.
AC Q12069; D6VRV8; Q6B2P8;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=tRNA pseudouridine(32) synthase, mitochondrial;
DE EC=5.4.99.28;
DE AltName: Full=tRNA pseudouridine synthase 9;
DE AltName: Full=tRNA pseudouridylate synthase 9;
DE AltName: Full=tRNA-uridine isomerase 9;
DE Flags: Precursor;
GN Name=PUS9; OrderedLocusNames=YDL036C; ORFNames=D2743;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBCELLULAR LOCATION.
RX PubMed=15466869; DOI=10.1074/jbc.m409581200;
RA Behm-Ansmant I., Grosjean H., Massenet S., Motorin Y., Branlant C.;
RT "Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs
RT requires two distinct enzymes in Saccharomyces cerevisiae.";
RL J. Biol. Chem. 279:52998-53006(2004).
RN [6]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=25219674; DOI=10.1016/j.cell.2014.08.028;
RA Schwartz S., Bernstein D.A., Mumbach M.R., Jovanovic M., Herbst R.H.,
RA Leon-Ricardo B.X., Engreitz J.M., Guttman M., Satija R., Lander E.S.,
RA Fink G., Regev A.;
RT "Transcriptome-wide mapping reveals widespread dynamic-regulated
RT pseudouridylation of ncRNA and mRNA.";
RL Cell 159:148-162(2014).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-32 in
CC mitochondrial transfer RNAs. {ECO:0000269|PubMed:15466869,
CC ECO:0000269|PubMed:25219674}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(32) in tRNA = pseudouridine(32) in tRNA;
CC Xref=Rhea:RHEA:42544, Rhea:RHEA-COMP:10107, Rhea:RHEA-COMP:10108,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.28;
CC Evidence={ECO:0000269|PubMed:15466869, ECO:0000269|PubMed:25219674};
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305|PubMed:15466869}.
CC -!- MISCELLANEOUS: Present with 639 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000305}.
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DR EMBL; Z71781; CAA96453.1; -; Genomic_DNA.
DR EMBL; Z74084; CAA98595.1; -; Genomic_DNA.
DR EMBL; AY692682; AAT92701.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11818.1; -; Genomic_DNA.
DR PIR; S67569; S67569.
DR RefSeq; NP_010248.1; NM_001180095.1.
DR AlphaFoldDB; Q12069; -.
DR SMR; Q12069; -.
DR BioGRID; 32022; 41.
DR DIP; DIP-5399N; -.
DR MINT; Q12069; -.
DR STRING; 4932.YDL036C; -.
DR iPTMnet; Q12069; -.
DR MaxQB; Q12069; -.
DR PaxDb; Q12069; -.
DR PRIDE; Q12069; -.
DR EnsemblFungi; YDL036C_mRNA; YDL036C; YDL036C.
DR GeneID; 851525; -.
DR KEGG; sce:YDL036C; -.
DR SGD; S000002194; PUS9.
DR VEuPathDB; FungiDB:YDL036C; -.
DR eggNOG; KOG1919; Eukaryota.
DR GeneTree; ENSGT00420000029802; -.
DR HOGENOM; CLU_016902_12_4_1; -.
DR InParanoid; Q12069; -.
DR OMA; VAYHDEM; -.
DR BioCyc; MetaCyc:G3O-29460-MON; -.
DR BioCyc; YEAST:G3O-29460-MON; -.
DR BRENDA; 5.4.99.28; 984.
DR PRO; PR:Q12069; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12069; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005739; C:mitochondrion; IMP:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0009982; F:pseudouridine synthase activity; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IDA:UniProtKB.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IDA:SGD.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006224; PsdUridine_synth_RluC/D_CS.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR TIGRFAMs; TIGR00005; rluA_subfam; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 1: Evidence at protein level;
KW Isomerase; Mitochondrion; Reference proteome; RNA-binding; Transit peptide;
KW tRNA processing.
FT TRANSIT 1..24
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 25..462
FT /note="tRNA pseudouridine(32) synthase, mitochondrial"
FT /id="PRO_0000162756"
FT DOMAIN 127..188
FT /note="S4 RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT ACT_SITE 238
FT /evidence="ECO:0000250|UniProtKB:P0AA37"
FT CONFLICT 458
FT /note="W -> R (in Ref. 3; AAT92701)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 462 AA; 53399 MW; 300E676D8E3819F0 CRC64;
MQRNNRLRNL FTVPVIMARQ LKRNALSAGL AFAGNATSNE FDEHLQNEVE REREIQKKKK
IKRTQSKKSP DLINKSTFQS RTIGSKKEKH RQLDPEYEIV IDGPLRKIKP YHFTYRTFCK
ERWRDKKLVD VFISEFRDRE SEYYKRTIEN GDVHINDETA DLSTVIRNGD LITHQVHRHE
PPVTSRPIKV IFEDDNIMVI DKPSGIPVHP TGRYRFNTIT KMLQNNLGFV VNPCNRLDRL
TSGLMFLAKT PKGADNIGDQ LKAREVTKEY VAKVVGEFPE TEVIVEKPLK LIEPRLALNA
VCQMDEKGAK HAKTVFNRIS YDGKTSIVKC KPLTGRSHQI RVHLQYLGHP IANDPIYSND
EVWGNNLGKG GQADFDIVIT KLDEIGKRKP AKSWFHSNGG YGEVLRQEKC SICESDLYTD
PGPNDLDLWL HAYLYESTET EEGTEKKKWC YKTEYPEWAL RR
