ID   PUSH_ARATH              Reviewed;         510 AA.
AC   O22928;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Putative tRNA pseudouridine synthase;
DE            EC=5.4.99.-;
DE   AltName: Full=tRNA pseudouridylate synthase;
DE   AltName: Full=tRNA-uridine isomerase;
GN   OrderedLocusNames=At2g30320; ORFNames=T09D09.13;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA = a pseudouridine in tRNA;
CC         Xref=Rhea:RHEA:54572, Rhea:RHEA-COMP:13339, Rhea:RHEA-COMP:13934,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000305}.
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DR   EMBL; AC002338; AAC16945.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC08370.1; -; Genomic_DNA.
DR   PIR; A84707; A84707.
DR   RefSeq; NP_180591.1; NM_128585.2.
DR   AlphaFoldDB; O22928; -.
DR   SMR; O22928; -.
DR   BioGRID; 2931; 2.
DR   STRING; 3702.AT2G30320.1; -.
DR   PaxDb; O22928; -.
DR   PRIDE; O22928; -.
DR   ProteomicsDB; 236542; -.
DR   EnsemblPlants; AT2G30320.1; AT2G30320.1; AT2G30320.
DR   GeneID; 817582; -.
DR   Gramene; AT2G30320.1; AT2G30320.1; AT2G30320.
DR   KEGG; ath:AT2G30320; -.
DR   Araport; AT2G30320; -.
DR   TAIR; locus:2065748; AT2G30320.
DR   eggNOG; KOG2553; Eukaryota.
DR   HOGENOM; CLU_023331_0_0_1; -.
DR   InParanoid; O22928; -.
DR   OMA; EAPWKEW; -.
DR   OrthoDB; 710785at2759; -.
DR   PhylomeDB; O22928; -.
DR   PRO; PR:O22928; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O22928; baseline and differential.
DR   Genevisible; O22928; AT.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR   CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR041708; PUS1/PUS2-like.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..510
FT                   /note="Putative tRNA pseudouridine synthase"
FT                   /id="PRO_0000057533"
FT   REGION          235..300
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        248..273
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        125
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         190
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   510 AA;  58972 MW;  F0995C47FF63AC82 CRC64;
     MAVSFLRIPI PSRVFWYPTR LIRVTPALLR FSSSAAFQPS STSLSPPSSD NFLADKWESY
     RKKKVVIRIG YVGTDYRGLQ IQRDDPSIKT IEGELEVAIY KAGGIRDSNY GDLHKIGWAR
     SSRTDKGVHS LATSISLKME IPETAWKDDP QGTVLAKCIS KHLPENIRVF SVLPSNRRFD
     PRRECTLRKY SYLLPVDVLG IKNSFTSDEI DYHITDFNEI LKEFEGEYPF HNYTQRSRYR
     RKSEQKIKQR NGRPPREPKS IKASESEFRE ENHIEIGEEE EEKEVDGESD EHVVTPDSDN
     SQVYSRAKWL YEPDETDKIS GAHFRKVFRC RSGKLENSLG FGFVEISIWG ESFMLHQIRK
     MIGTAVAVKR ELLPRDIIRL SLNKFTRIVL PLAPSEVLIL RGNSFEVRRL PERPGMEATG
     ESEEVEKEIE EFYRAVMVPQ VSIFLDSEKS PWKEWVDHLD RNDGLIDEEL EDVRKGWEEW
     KAAKPWMNLK KTEDDEEALS SVSVPIHQAL