PUSL1_MOUSE
ID PUSL1_MOUSE Reviewed; 291 AA.
AC A2ADA5; Q3TVB3; Q3UAA3;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=tRNA pseudouridine synthase-like 1;
DE EC=5.4.99.-;
DE AltName: Full=tRNA pseudouridylate synthase-like 1;
DE AltName: Full=tRNA-uridine isomerase-like 1;
GN Name=Pusl1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in tRNA = a pseudouridine in tRNA;
CC Xref=Rhea:RHEA:54572, Rhea:RHEA-COMP:13339, Rhea:RHEA-COMP:13934,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A2ADA5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A2ADA5-2; Sequence=VSP_036050, VSP_036051, VSP_036053;
CC Name=3;
CC IsoId=A2ADA5-3; Sequence=VSP_036049, VSP_036052, VSP_036054;
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000305}.
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DR EMBL; AK151452; BAE30412.1; -; mRNA.
DR EMBL; AK160236; BAE35706.1; -; mRNA.
DR EMBL; AL670236; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS51404.1; -. [A2ADA5-1]
DR RefSeq; NP_001028662.1; NM_001033490.1. [A2ADA5-1]
DR RefSeq; XP_006539075.1; XM_006539012.1.
DR AlphaFoldDB; A2ADA5; -.
DR SMR; A2ADA5; -.
DR BioGRID; 241456; 2.
DR STRING; 10090.ENSMUSP00000095344; -.
DR PhosphoSitePlus; A2ADA5; -.
DR EPD; A2ADA5; -.
DR MaxQB; A2ADA5; -.
DR PaxDb; A2ADA5; -.
DR PeptideAtlas; A2ADA5; -.
DR PRIDE; A2ADA5; -.
DR ProteomicsDB; 300358; -. [A2ADA5-1]
DR ProteomicsDB; 300359; -. [A2ADA5-2]
DR ProteomicsDB; 300360; -. [A2ADA5-3]
DR Antibodypedia; 26254; 75 antibodies from 17 providers.
DR Ensembl; ENSMUST00000097737; ENSMUSP00000095344; ENSMUSG00000051557. [A2ADA5-1]
DR GeneID; 433813; -.
DR KEGG; mmu:433813; -.
DR UCSC; uc008wfi.1; mouse. [A2ADA5-2]
DR UCSC; uc012drb.1; mouse. [A2ADA5-1]
DR CTD; 126789; -.
DR MGI; MGI:3047787; Pusl1.
DR VEuPathDB; HostDB:ENSMUSG00000051557; -.
DR eggNOG; KOG4393; Eukaryota.
DR GeneTree; ENSGT00950000183160; -.
DR HOGENOM; CLU_014673_3_1_1; -.
DR InParanoid; A2ADA5; -.
DR OMA; FLYGMVR; -.
DR OrthoDB; 831280at2759; -.
DR PhylomeDB; A2ADA5; -.
DR TreeFam; TF105127; -.
DR BioGRID-ORCS; 433813; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Pusl1; mouse.
DR PRO; PR:A2ADA5; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; A2ADA5; protein.
DR Bgee; ENSMUSG00000051557; Expressed in embryonic brain and 63 other tissues.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; ISO:MGI.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IBA:GO_Central.
DR CDD; cd02570; PseudoU_synth_EcTruA; 1.
DR Gene3D; 3.30.70.580; -; 1.
DR Gene3D; 3.30.70.660; -; 1.
DR HAMAP; MF_00171; TruA; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142; PTHR11142; 1.
DR Pfam; PF01416; PseudoU_synth_1; 2.
DR PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Isomerase; Reference proteome; tRNA processing.
FT CHAIN 1..291
FT /note="tRNA pseudouridine synthase-like 1"
FT /id="PRO_0000358316"
FT ACT_SITE 66
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT BINDING 130
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1..65
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_036049"
FT VAR_SEQ 1..35
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_036050"
FT VAR_SEQ 36..45
FT /note="RAVGVLNFLE -> MWQPPVRFPQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_036051"
FT VAR_SEQ 66..69
FT /note="DAGV -> MPGL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_036052"
FT VAR_SEQ 232
FT /note="R -> RQVGCLNPHRMGSGRHTCDNLSSLL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_036053"
FT VAR_SEQ 266..291
FT /note="KYQARVAPARGLFLKSVLYDNFGPTS -> QVSGSSCSSPWPVLEVSAV
FT (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_036054"
SQ SEQUENCE 291 AA; 31993 MW; C7013FBEE8318494 CRC64;
MGSCGAVGSV RARYLVFLQY LGTDFNGVAA VRGNPRAVGV LNFLEEAAKR LNSVDPVRFT
ISSRTDAGVH ALSNAAHLDI QRRPGQSPFS PEVVAKALNT HLKHPAIRVL KAFRVPNDFH
ARHAATSRTY QYRLATGCSW PNQLPVFEQN VCWALQTEYL DMAAMQEAAQ HLLGTHDFSA
FQSAGSPVTN TVRTLRRVSV SPGPASPFVL PEGSRRLQFW TLEFESQSFL YRQVRRMTAV
LVAVGLGILA PTQVKVILES QDPLGKYQAR VAPARGLFLK SVLYDNFGPT S
