ID   PUSL1_XENLA             Reviewed;         298 AA.
AC   Q6GQ53;
DT   16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=tRNA pseudouridine synthase-like 1;
DE            EC=5.4.99.-;
DE   AltName: Full=tRNA pseudouridylate synthase-like 1;
DE   AltName: Full=tRNA-uridine isomerase-like 1;
GN   Name=pusl1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in tRNA = a pseudouridine in tRNA;
CC         Xref=Rhea:RHEA:54572, Rhea:RHEA-COMP:13339, Rhea:RHEA-COMP:13934,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC072895; AAH72895.1; -; mRNA.
DR   RefSeq; NP_001085529.1; NM_001092060.1.
DR   AlphaFoldDB; Q6GQ53; -.
DR   SMR; Q6GQ53; -.
DR   DNASU; 443955; -.
DR   GeneID; 443955; -.
DR   KEGG; xla:443955; -.
DR   CTD; 443955; -.
DR   Xenbase; XB-GENE-991886; pusl1.L.
DR   OrthoDB; 831280at2759; -.
DR   Proteomes; UP000186698; Chromosome 7L.
DR   Bgee; 443955; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   CDD; cd02570; PseudoU_synth_EcTruA; 1.
DR   Gene3D; 3.30.70.580; -; 1.
DR   Gene3D; 3.30.70.660; -; 1.
DR   HAMAP; MF_00171; TruA; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR001406; PsdUridine_synth_TruA.
DR   InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR   InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR   InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR   PANTHER; PTHR11142; PTHR11142; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 1.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; Reference proteome; tRNA processing.
FT   CHAIN           1..298
FT                   /note="tRNA pseudouridine synthase-like 1"
FT                   /id="PRO_0000358317"
FT   ACT_SITE        60
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         124
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   298 AA;  33683 MW;  1F0DF6A743DBABA5 CRC64;
     MHSSKARYLV FFQYYGTKYS GVMETPITQS VLGVQNYLEV AAQKLRPVGT IKFFISSRTD
     SGVHAICNLA HVDIERAKGK PPFTEEILVQ AMNHHLQPEP IRLLKAIRVC DNFHARHNAL
     SRTYVYRVAA GCSRPELPVF EGNLCWAIQD GLNVTAINEA SKMLLGTHNF SAFRSANSEN
     AFKSPIKTLQ QADINPSCGI LTHHWQSRNL QFWDFTFRAR SFLYKQVRRM TGALVAVGQG
     RLTPQQIKEF LENQDPNCFL GNFVAPPHGL FLKDVEYDGT ELNGFSAEET QKYSSHLN