PUT1_ORYSJ
ID PUT1_ORYSJ Reviewed; 531 AA.
AC Q6Z8D0; A0A0P0VNK8;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Polyamine transporter PUT1;
DE AltName: Full=Polyamine uptake transporter 1;
DE Short=OsPUT1;
GN Name=PUT1; OrderedLocusNames=Os02g0700500, LOC_Os02g47210;
GN ORFNames=OJ1111_E07.11, OsJ_08051, P0459B01.40;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=21796369; DOI=10.1007/s00425-011-1486-9;
RA Mulangi V., Phuntumart V., Aouida M., Ramotar D., Morris P.;
RT "Functional analysis of OsPUT1, a rice polyamine uptake transporter.";
RL Planta 235:1-11(2012).
CC -!- FUNCTION: Cell membrane polyamine/proton symporter involved in the
CC polyamine uptake in cells. Possesses high affinity for spermidine and
CC lower affinity for spermine and putrescine. Transports paraquat, a
CC polyamine analog, and thus confers sensitivity to this chemical which
CC is used as a herbicide. {ECO:0000269|PubMed:21796369}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=15.2 uM for spermidine {ECO:0000269|PubMed:21796369};
CC Vmax=12 pmol/min/mg enzyme toward spermidine
CC {ECO:0000269|PubMed:21796369};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:21796369};
CC Multi-pass membrane protein {ECO:0000305|PubMed:21796369}. Note=Plasma
CC membrane.
CC -!- TISSUE SPECIFICITY: Expressed in seedling roots, leaves, stems, flowers
CC and siliques. {ECO:0000269|PubMed:21796369}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP003994; BAD07518.1; -; Genomic_DNA.
DR EMBL; AP004778; BAD07889.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09754.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS80462.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ24299.1; -; Genomic_DNA.
DR EMBL; AK068055; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; Q6Z8D0; -.
DR SMR; Q6Z8D0; -.
DR STRING; 4530.OS02T0700500-01; -.
DR TCDB; 2.A.3.12.5; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; Q6Z8D0; -.
DR PRIDE; Q6Z8D0; -.
DR EnsemblPlants; Os02t0700500-01; Os02t0700500-01; Os02g0700500.
DR Gramene; Os02t0700500-01; Os02t0700500-01; Os02g0700500.
DR eggNOG; KOG1287; Eukaryota.
DR HOGENOM; CLU_007946_17_3_1; -.
DR InParanoid; Q6Z8D0; -.
DR OMA; PLIWIWL; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6Z8D0; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015203; F:polyamine transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015846; P:polyamine transport; IDA:UniProtKB.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR044566; RMV1-like.
DR PANTHER; PTHR45826; PTHR45826; 1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..531
FT /note="Polyamine transporter PUT1"
FT /id="PRO_0000418913"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 391..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 414..434
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 476..496
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 531 AA; 56626 MW; BAE56BA6CD271EFE CRC64;
MADTGGRPEV SLATVRSPGH PAASTTAAAA ADLGHADTGQ EKPTVESAQP ANGAAPMGEC
GTEYRGLPDG DAGGPMPSSA RTVSMIPLIF LIFYEVSGGP FGIEDSVGAA GPLLAIIGFL
VLPVIWSIPE ALITAELGAM FPENGGYVVW VASALGPYWG FQQGWMKWLS GVIDNALYPV
LFLDYLKSGV PALGGGAPRA FAVVGLTAVL TLLNYRGLTV VGWVAICLGV FSLLPFFVMG
LIALPKLRPA RWLVIDLHNV DWNLYLNTLF WNLNYWDSIS TLAGEVKNPG KTLPKALFYA
VIFVVVAYLY PLLAGTGAVP LDRGQWTDGY FADIAKLLGG AWLMWWVQSA AALSNMGMFV
AEMSSDSYQL LGMAERGMLP SFFAARSRYG TPLAGILFSA SGVLLLSMMS FQEIVAAENF
LYCFGMLLEF VAFILHRVRR PDAARPYRVP LGTAGCVAML VPPTALIAVV LALSTLKVAV
VSLGAVAMGL VLQPALRFVE KKRWLRFSVN PDLPEIGVIR PPAAPDEPLV P
