ID   PUTDT_BPW14             Reviewed;         310 AA.
AC   C9DG80;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=Putrescinyltransferase {ECO:0000305};
DE   AltName: Full=Amino acid:DNA transferase {ECO:0000303|PubMed:34522950};
DE            Short=AADT {ECO:0000303|PubMed:34522950};
GN   Name=gp109 {ECO:0000305};
GN   and
GN   Name=109 {ECO:0000312|EMBL:ACV50131.1};
OS   Delftia phage PhiW-14 (Deftia acidovorans bacteriophage phiW-14).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Ionavirus; Delftia virus PhiW14.
OX   NCBI_TaxID=665032;
OH   NCBI_TaxID=80866; Delftia acidovorans (Pseudomonas acidovorans) (Comamonas acidovorans).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kropinski A.M., Villegas A., Lingohr E.J.;
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION.
RC   STRAIN=Mutant am37 {ECO:0000305};
RX   PubMed=7260058; DOI=10.1021/bi00515a043;
RA   Maltman K.L., Neuhard J., Warren R.A.;
RT   "5-[(Hydroxymethyl)-O-pyrophosphoryl]uracil, an intermediate in the
RT   biosynthesis of alpha-putrescinylthymine in deoxyribonucleic acid of
RT   bacteriophage phi W-14.";
RL   Biochemistry 20:3586-3591(1981).
RN   [3]
RP   FUNCTION.
RC   STRAIN=Mutant am36, and Mutant am42;
RX   PubMed=7109031; DOI=10.1128/jvi.43.1.67-72.1982;
RA   Miller P.B., Maltman K.L., Warren R.A.;
RT   "Isolation and preliminary characterization of amber mutants of
RT   bacteriophage phi W-14 defective in DNA synthesis.";
RL   J. Virol. 43:67-72(1982).
RN   [4]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=34522950; DOI=10.1093/nar/gkab781;
RA   Lee Y.J., Dai N., Mueller S.I., Guan C., Parker M.J., Fraser M.E.,
RA   Walsh S.E., Sridar J., Mulholland A., Nayak K., Sun Z., Lin Y.C.,
RA   Comb D.G., Marks K., Gonzalez R., Dowling D.P., Bandarian V., Saleh L.,
RA   Correa I.R., Weigele P.R.;
RT   "Pathways of thymidine hypermodification.";
RL   Nucleic Acids Res. 0:0-0(2021).
CC   -!- FUNCTION: Transfers putrescine to 5-phosphomethyl-2'-deoxyuridine (5-
CC       PmdU) to produce 5-Nalpha-putrescinylthymidine (Nalpha-PutT) as a step
CC       in the pathway leading to thymidine hypermodifications in the viral
CC       genome (PubMed:34522950, PubMed:7260058, PubMed:7109031). As a final
CC       result of the pathway of hypermodification, Nalpha-PutT substitutes for
CC       about 50% of thymidines in the viral DNA (PubMed:7260058). These
CC       modifications probably prevent degradation of viral genome by the host
CC       restriction-modification antiviral defense system (PubMed:34522950).
CC       {ECO:0000269|PubMed:34522950, ECO:0000269|PubMed:7109031,
CC       ECO:0000269|PubMed:7260058}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-phosphomethyl-dUMP in DNA + putrescine = 5-N(alpha)-
CC         putrescinyl-dTMP in DNA + phosphate; Xref=Rhea:RHEA:71575, Rhea:RHEA-
CC         COMP:18039, Rhea:RHEA-COMP:18045, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:190918, ChEBI:CHEBI:190920, ChEBI:CHEBI:326268;
CC         Evidence={ECO:0000269|PubMed:34522950};
CC   -!- MISCELLANEOUS: Mutant am37 contains a normal amount of thymine, no
CC       alpha-putrescinylthymine, and hydroxymethyluracil (PubMed:7260058,
CC       PubMed:7109031). Mutants am36 and am42 contain more thymine and less a-
CC       putrescinylthymine than wild-type (PubMed:7109031).
CC       {ECO:0000269|PubMed:7109031, ECO:0000269|PubMed:7260058}.
CC   -!- SIMILARITY: Belongs to the thymidine aminotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; GQ357915; ACV50131.1; -; Genomic_DNA.
DR   RefSeq; YP_003358963.1; NC_013697.1.
DR   GeneID; 8684057; -.
DR   KEGG; vg:8684057; -.
DR   Proteomes; UP000008986; Genome.
DR   InterPro; IPR040741; Alpha_GPT-Pplase2.
DR   Pfam; PF18724; aGPT-Pplase2; 1.
PE   1: Evidence at protein level;
KW   Host-virus interaction; Reference proteome;
KW   Restriction-modification system evasion by virus; Transferase.
FT   CHAIN           1..310
FT                   /note="Putrescinyltransferase"
FT                   /id="PRO_0000456275"
SQ   SEQUENCE   310 AA;  35268 MW;  044A81372F539EE3 CRC64;
     MKHSYRADEP LWLNYTEAIS KTWDIDPTYN AIHGAMKDMG EAKTHRVLAG MALYYHLGFS
     CQLAEQSTDE NFWDVLGNNY EGTKRGTERR YFRGEQGRAS LKYIKDNYKT PTDFLLGLHR
     DKYSDLLKAF GPVPAFGPYF VWKMGDIYDR ILGMPIQADN CIEHLPSEPF KGMELVRKEM
     IARGDASFAD YTPQQMFEYM ESQTNKLGLI APPAGDRLLD IREIETAMCG LKHFYTGTDY
     VGKDLVKHSE SLLGYGETAD LLRSHMPPVI ARDYFMAPAS VIKLHGPNRK IDEEGSNPIV
     KTTGLGSFFR