ID   PUTP1_STAS1             Reviewed;         514 AA.
AC   Q4A070;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Sodium/proline symporter 1;
DE   AltName: Full=Proline permease 1;
GN   Name=putP1; OrderedLocusNames=SSP0399;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC       proline. {ECO:0000250|UniProtKB:P07117}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC         Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC         Evidence={ECO:0000250|UniProtKB:P07117};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
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DR   EMBL; AP008934; BAE17544.1; -; Genomic_DNA.
DR   RefSeq; WP_011302372.1; NZ_MTGA01000036.1.
DR   AlphaFoldDB; Q4A070; -.
DR   SMR; Q4A070; -.
DR   STRING; 342451.SSP0399; -.
DR   EnsemblBacteria; BAE17544; BAE17544; SSP0399.
DR   KEGG; ssp:SSP0399; -.
DR   PATRIC; fig|342451.11.peg.404; -.
DR   eggNOG; COG0591; Bacteria.
DR   HOGENOM; CLU_018808_15_2_9; -.
DR   OMA; AWKTKNT; -.
DR   OrthoDB; 231452at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005298; F:proline:sodium symporter activity; IEA:InterPro.
DR   GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR   GO; GO:0015824; P:proline transport; IEA:InterPro.
DR   CDD; cd11475; SLC5sbd_PutP; 1.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR011851; Na/Pro_symporter.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   Pfam; PF00474; SSF; 1.
DR   TIGRFAMs; TIGR02121; Na_Pro_sym; 1.
DR   TIGRFAMs; TIGR00813; sss; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..514
FT                   /note="Sodium/proline symporter 1"
FT                   /id="PRO_0000364109"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        144..164
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        204..224
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        438..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        467..487
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   514 AA;  55615 MW;  B65E65CF782931D7 CRC64;
     MFTLGTSLSS QVDPNWQTYI MLVVYFVVLL VIGYYGFKQS TGNVSEYMLG GRNIGPYVTA
     LSAGASDMSG WMIMGLPGEV YTTGLSAAWL AIGLTLGAYI NYIVVAPRLR VYTEKAGDAI
     TLPDFFKNRL DDKSNSIKII SGAIIVVFFT LYTHSGMVSG GVLFESAFGV NYHIGMLLVA
     VIVIAYTFFG GYLAVSLTDF FQGVVMIIAM VMVPIVAMLQ LSGLDTFTQT AELKPTNLDL
     FKGTTVIGII SFFAWGLGYF GQPHIIVRFM SIKSVKQLPT ARRFGIGWMA ISLLGAVGVG
     LTGITFINQS GTDIENPETL FVLMGQILFH PLVGGFLLAA ILAAIMSTIS SQLLVTSSSL
     TEDFYKLIRG EEAAKEHEKE FVLVGRLSVI IVAIVSIWIA WSPNDTILGL VGNAWAGFGA
     AFGPLVLLSL YWKGLSRTGA VSGMLSGAIV VIIWIAFVKP LGDVNDFFNL YEIIPGFLTS
     LIVTVVVSKF TKKPQIDVEA DLTDVRRLVK TGED