ID   PUTP2_STAS1             Reviewed;         511 AA.
AC   Q49YU6;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Sodium/proline symporter 2;
DE   AltName: Full=Proline permease 2;
GN   Name=putP2; OrderedLocusNames=SSP0889;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
CC   -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC       proline. {ECO:0000250|UniProtKB:P07117}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC         Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC         Evidence={ECO:0000250|UniProtKB:P07117};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
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DR   EMBL; AP008934; BAE18034.1; -; Genomic_DNA.
DR   RefSeq; WP_011302766.1; NZ_MTGA01000031.1.
DR   AlphaFoldDB; Q49YU6; -.
DR   SMR; Q49YU6; -.
DR   STRING; 342451.SSP0889; -.
DR   EnsemblBacteria; BAE18034; BAE18034; SSP0889.
DR   KEGG; ssp:SSP0889; -.
DR   PATRIC; fig|342451.11.peg.888; -.
DR   eggNOG; COG0591; Bacteria.
DR   HOGENOM; CLU_018808_15_2_9; -.
DR   OMA; CWTDLIQ; -.
DR   OrthoDB; 231452at2; -.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005298; F:proline:sodium symporter activity; IEA:InterPro.
DR   GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR   GO; GO:0015824; P:proline transport; IEA:InterPro.
DR   CDD; cd11475; SLC5sbd_PutP; 1.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR011851; Na/Pro_symporter.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   Pfam; PF00474; SSF; 1.
DR   TIGRFAMs; TIGR02121; Na_Pro_sym; 1.
DR   TIGRFAMs; TIGR00813; sss; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Ion transport; Membrane;
KW   Reference proteome; Sodium; Sodium transport; Symport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..511
FT                   /note="Sodium/proline symporter 2"
FT                   /id="PRO_0000364110"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        204..224
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        246..266
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        438..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        467..487
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   511 AA;  56205 MW;  8B1607C32696E4E3 CRC64;
     MLILGTSLAN QVHASWQTYI MIIIYFTILL FIGYYGYKQA TGNLSEFMLG GRSIGPYVTA
     LSAGASDMSG WMIMGLPGSV YSTGLSAMWI TIGLSLGAYV NYFVVAPRLR VYTELAGDAI
     TLPDFFKNRL NDHNNYIKII SGLIIVVFFT LYTHSGFVSG GKLFESAFGL NYHWGLLMVA
     FIVIFYTFFG GYLAVSITDF FQGVIMLIAM VMVPIVALID LNGIDTFKQV AEMKPTNMNL
     FKGTTVLGII SLFAWGLGYF GQPHIIVRFM SIKSHKLLPK ARRLGISWMV IGLLGAVAVG
     LTGIAFISER NIKLEDPETL FIVMSQILFH PLVGGFLLAA ILAAIMSTIS SQLLVTSSSL
     TEDFYKLIRG EDKAKAHEKE FLMVGRLSVL IVAIVAIWIA WSPNDTILNL VGNAWAGFGA
     AFSPLVIFSL YWKGLSRTGA LAGMITGALV VIIWIVWIKP LASINELFGM YEIIPGFLAS
     VITTYFVSKY TKKPGSFVTH DLDKVKQIVK E