ID   PUTP_STAAM              Reviewed;         512 AA.
AC   Q99SY5;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Sodium/proline symporter;
DE   AltName: Full=Proline permease;
GN   Name=putP; OrderedLocusNames=SAV1902;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
CC   -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC       proline (By similarity). Since most S.aureus strains are L-proline
CC       auxotrophs, this transporter may aid the bacterial persistence during
CC       an infection of tissues with low proline concentrations (By
CC       similarity). {ECO:0000250|UniProtKB:P07117,
CC       ECO:0000250|UniProtKB:Q2FWY7}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC         Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC         Evidence={ECO:0000250|UniProtKB:P07117};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
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DR   EMBL; BA000017; BAB58064.1; -; Genomic_DNA.
DR   RefSeq; WP_000957020.1; NC_002758.2.
DR   AlphaFoldDB; Q99SY5; -.
DR   SMR; Q99SY5; -.
DR   PaxDb; Q99SY5; -.
DR   EnsemblBacteria; BAB58064; BAB58064; SAV1902.
DR   KEGG; sav:SAV1902; -.
DR   HOGENOM; CLU_018808_15_2_9; -.
DR   OMA; CWTDLIQ; -.
DR   PhylomeDB; Q99SY5; -.
DR   BioCyc; SAUR158878:SAV_RS10420-MON; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005298; F:proline:sodium symporter activity; IEA:InterPro.
DR   GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR   GO; GO:0015824; P:proline transport; IEA:InterPro.
DR   CDD; cd11475; SLC5sbd_PutP; 1.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR011851; Na/Pro_symporter.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   Pfam; PF00474; SSF; 1.
DR   TIGRFAMs; TIGR02121; Na_Pro_sym; 1.
DR   TIGRFAMs; TIGR00813; sss; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Ion transport; Membrane; Sodium;
KW   Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..512
FT                   /note="Sodium/proline symporter"
FT                   /id="PRO_0000364099"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        200..220
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        286..306
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        438..458
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        467..487
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   512 AA;  55976 MW;  C0B4AC52311A96DD CRC64;
     MLTMGTALSQ QVDANWQTYI MIAVYFLILI VIGFYGYKQA TGNLSEYMLG GRSIGPYITA
     LSAGASDMSG WMIMGLPGSV YSTGLSAMWI TIGLTLGAYI NYFVVAPRLR VYTELAGDAI
     TLPDFFKNRL NDKNNVLKII SGLIIVVFFT LYTHSGFVSG GKLFESAFGL DYHFGLILVA
     FIVIFYTFFG GYLAVSITDF FQGVIMLIAM VMVPIVAMMN LNGWGTFHDV AAMKPTNLNL
     FKGLSFIGII SLFSWGLGYF GQPHIIVRFM SIKSHKMLPK ARRLGISWMA VGLLGAVAVG
     LTGIAFVPAY HIKLEDPETL FIVMSQVLFH PLVGGFLLAA ILAAIMSTIS SQLLVTSSSL
     TEDFYKLIRG EEKAKTHQKE FVMIGRLSVL VVAIVAIAIA WNPNDTILNL VGNAWAGFGA
     SFSPLVLFAL YWKGLTRAGA VSGMVSGALV VIVWIAWIKP LAHINEIFGL YEIIPGFIVS
     VIVTYVVSKL TKKPGAFVET DLNKVRDIVR EK