ID   PUTP_STAAU              Reviewed;         511 AA.
AC   Q53584;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Sodium/proline symporter;
DE   AltName: Full=Proline permease {ECO:0000303|PubMed:7887605};
GN   Name=putP {ECO:0000303|PubMed:7887605};
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 12600 / DSM 20231 / IAM 12544 / NCDO 949 / NCTC 8532;
RX   PubMed=7887605; DOI=10.1128/aem.61.1.252-259.1995;
RA   Wengender P.A., Miller K.J.;
RT   "Identification of a putP proline permease gene homolog from Staphylococcus
RT   aureus by expression cloning of the high-affinity proline transport system
RT   in Escherichia coli.";
RL   Appl. Environ. Microbiol. 61:252-259(1995).
CC   -!- FUNCTION: Catalyzes the sodium-dependent uptake of extracellular L-
CC       proline (Probable). Since most S.aureus strains are L-proline
CC       auxotrophs, this transporter may aid the bacterial persistence during
CC       an infection of tissues with low proline concentrations (By
CC       similarity). {ECO:0000250|UniProtKB:Q2FWY7,
CC       ECO:0000305|PubMed:7887605}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-proline(in) + Na(+)(in) = L-proline(out) + Na(+)(out);
CC         Xref=Rhea:RHEA:28967, ChEBI:CHEBI:29101, ChEBI:CHEBI:60039;
CC         Evidence={ECO:0000250|UniProtKB:P07117};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA79365.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; U06451; AAA79365.1; ALT_FRAME; Genomic_DNA.
DR   AlphaFoldDB; Q53584; -.
DR   SMR; Q53584; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005298; F:proline:sodium symporter activity; IEA:InterPro.
DR   GO; GO:0031402; F:sodium ion binding; IEA:InterPro.
DR   GO; GO:0015824; P:proline transport; IEA:InterPro.
DR   CDD; cd11475; SLC5sbd_PutP; 1.
DR   Gene3D; 1.20.1730.10; -; 1.
DR   InterPro; IPR038377; Na/Glc_symporter_sf.
DR   InterPro; IPR011851; Na/Pro_symporter.
DR   InterPro; IPR001734; Na/solute_symporter.
DR   Pfam; PF00474; SSF; 1.
DR   TIGRFAMs; TIGR02121; Na_Pro_sym; 1.
DR   TIGRFAMs; TIGR00813; sss; 1.
DR   PROSITE; PS50283; NA_SOLUT_SYMP_3; 1.
PE   3: Inferred from homology;
KW   Amino-acid transport; Cell membrane; Ion transport; Membrane; Sodium;
KW   Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..511
FT                   /note="Sodium/proline symporter"
FT                   /id="PRO_0000364091"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        84..104
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        173..193
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        285..305
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        326..346
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..400
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        409..429
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        437..457
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        466..486
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   511 AA;  55880 MW;  D932E318275D64F9 CRC64;
     MLTMGTALSQ QVDANWQTYI MIAVYFLILM LLAFTYKQAT GNLSEYMLGG RSIGPYITAL
     SAGASDMSGW MIMGLPGSVY STGLSAMWIT IGLTLGAYIN YFVVAPRLRV YTELAGDAIT
     LPDFFKNRLN DKNNVLKIIS GLIIVVFFTL YTHSGFVSGG KLFESAFGLD YHFGLILVAF
     IVIFYTFFGG YLAVSITDFF QGVIMLIAMV MVPIVAMMNL NGWGTFHDVA AMKPTNLNLF
     KGLSFIGIIS LFSWGLGYFG QPHIIVRFMS IKSHKMLPKA RRLGISWMAV GLLGAVAVGL
     TGIAFVPAYH IKLEDPETLF IVMSQVLFHP LVGGFLLAAI LAAIMSTISS QLLVTSSSLT
     EDFYKLIRGE EKAKTDQKEF VMIGRLSVLV VAIVAIAIAW NPNDTILNLV GNAWAGFGAS
     FSPLVLFALY WKGLTRAGAV SGMVSGALVV IVWIAWIKPL AHINEIFGLY EIIPGFIVSV
     IVTYVVSKLT KKPGAFVETD LNKVRDIVRE K